Korean J Asthma Allergy Clin Immunol.  2010 Sep;30(3):209-215.

Measurement Specific IgE against Recombinant 16-kD and 19-kD Buckwheat Allergens for the Diagnosis of Buckwheat Allergy

Abstract

BACKGROUND
Buckwheat (BW, Fagopyrum esculentum)-specific IgE (sIgE) measurement is crucial to diagnose BW allergy. However, clinical application is difficult due to a high false-positive rate. Previously we identified 16-kD 2S storage albumin and 19-kD vicilin-like protein as BW major allergens.
OBJECTIVE
To investigate clinical usefulness of sIgE measurements against recombinant 16- and 19-kD BW allergens compared to the skin prick test and measurement of specific IgE against whole buckwheat (WBW) extract. METHOD: Eleven BW allergic patients, 11 asymptomatic sensitizers to BW and 16 non-atopic subjects were enrolled. We performed the skin prick test, sIgE measurement against recombinant 16- and 19-kD BW allergens and WBW extract using a CAP system. RESULT: In BW allergic patients, sIgE against WBW extract, r16- and r19-kD BW allergens were detected in 10, 5 and 9 patients, respectively. In asymptomatic sensitizers, sIgE against WBW extract, r16- and r19-kD BW allergens were detected in 9, 1 and 2 patients respectively. In ROC analysis, the AUC value of sIgE level was higher in r19-kD allergen than in r16-kD or WBW extract.
CONCLUSION
We suggest that CAP sIgE against r19-kD and r16-kD BW allergens can be a diagnostic marker to discriminate BW allergic patients from asymptomatic sensitizers in a clinical setting.

MeSH Terms

Allergens
Area Under Curve
Fagopyrum
Humans
Hypersensitivity
Immunoglobulin E
ROC Curve
Skin
Staphylococcal Protein A
Allergens
Immunoglobulin E
Staphylococcal Protein A