Korean J Urol.  2006 Jun;47(6):607-613. 10.4111/kju.2006.47.6.607.

Correlation of Caveolin-1 Expression on Tissue Microarray with Prognosis in Renal Cell Carcinoma

Affiliations
  • 1Department of Urology, Ajou University School of Medicine, Suwon, Korea. sejoong@ajou.ac.kr
  • 2Department of Pathology, Ajou University School of Medicine, Suwon, Korea.

Abstract

PURPOSE: We investigated the relationship between the expression of caveolin-1, using a tissue microarray (TMA), and the prognosis of patients with renal cell carcinoma (RCC).
MATERIALS AND METHODS
TMA sections of formalin-fixed, paraffin-embedded tissues of RCC from 82 patients, who had undergone radical nephrectomy, were stained immunohistochemically with specific antibodies against caveolin-1. The caveolin-1 immunostaining was semi-quantitatively estimated based on intensity. The expression pattern of caveolin-1 was compared with the clinicopathological variables.
RESULTS
The expression of caveolin-1 was significantly correlated with tumor size (p=0.002), TNM stage (p<0.001), T stage (p=0.001), M stage (p=0.004), grade (p=0.028) and metastasis (p<0.001), and was also significantly higher in clear cell than non-clear cell RCC (p<0.001). The survival of patients with higher caveolin-1 expression was significantly worse than that of patients with lower caveolin-1 expression (p=0.001). Univariate analyses were able to identify all variables, including caveolin-1 expression as significant prognostic factors for cancer-specific survival; multivariate analyses indicated that TNM stage, M stage and grade were independent prognostic factors. Caveolin-1 expression was not an independent factor.
CONCLUSIONS
The increased expression of caveolin-1 is associated with tumor size, stage, grade, metastasis and a worse prognosis in RCC, which suggests that caveolin-1 may be important in the progression of RCC.

Keyword

Caveolin-1; Tissue microarray; Prognosis; Renal cell carcinoma

MeSH Terms

Antibodies
Carcinoma, Renal Cell*
Caveolin 1*
Humans
Multivariate Analysis
Neoplasm Metastasis
Nephrectomy
Prognosis*
Antibodies
Caveolin 1

Figure

  • Fig. 1 Tissue microarray (TMA) slide of renal cell carcinoma tissues. (A) TMA paraffin block of renal cell carcinoma. (B) Immunohistochemically stained TMA slide (×40).

  • Fig. 2 Immunohistochemical staining for caveolin-1 in renal cell carcinoma tissues (×400). (A) Endothelial cells are stained as an internal control, but cancer cells are not stained (caveolin-1 intensity 0). (B) Cancer cells are strongly stained for caveolin-1 (intensity 3).

  • Fig. 3 Kaplan-Meier cancer-specific survival curves according to the expression of caveolin-1. The survival of patients with higher caveolin-1 expression is significantly worse than that of patients with lower caveolin-1 expression (p=0.001).


Cited by  1 articles

Caveolin-1 and Ki-67 Expression as Prognostic Factors in Clear Cell Carcinoma of the Kidney
Byung Hoon Kim, Chun Il Kim, Choal Hee Park
Korean J Urol. 2008;49(2):99-106.    doi: 10.4111/kju.2008.49.2.99.


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