Korean J Urol.
1989 Jun;30(3):301-306.
Colony Formation Assay and Chemosensitivity Test for Urologic Malignancies: A Preliminary Report
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- 1Soon Chun Hyang Medical College, Seoul, Korea.
Abstract
- Use of the Hamburger-Salmon soft agar assay method for in vitro chemotherapy sensitivity testing was tried. In this assay, double soft agar was used to culture 9 human urologic tumors, including 2 renal adenocarcinomas, one Wilms' tumor, 4 bladder transitional cell carcinomas, one paratesticular rhabdomyosarcoma and one penile squamous cell carcinoma. Among these cancers, Wilms' tumor and penile carcinoma grew to the extent that they could be used in chemosensitivity testing in soft agar (> or =30 colonies per control plate). It is concluded from this study that in vitro chemosensitivity testing by colony formation assay may be a useful tool in the treatment of urologic cancers, but more experiences should be gathered.
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