Korean J Physiol Pharmacol.
1998 Aug;2(4):471-477.
Acute cyclosporin A-treatment impairs the cytosolic guanylate
cyclase-mediated vasodilatation in rat thoracic aorta
- Affiliations
-
- 1Department of Pharmacology, Chonnam University Medical School, 5 Hak-dong, Dong-ku, Kwansiu 501-190, South Korea.
Abstract
- Cyclosporin A (CsA), a widely used immunosuppressant, is well known to
cause nephrotoxicity and hypertension as major side effects. The
present study was aimed at investigating the effects of
CsA-pretreatment on the activities of cytosolic guanylate cyclase (cGC)
in relation to the alteration of relaxant responses in the rat thoracic
aorta. CsA (10 micrometer)-preincubation for 90 min significantly attenuated
the vasodilatation induced by sodium nitroprusside (SNP), a cytosolic
guanylate cyclase activator, shifting the dose-response curve to the
right. The increase in cGMP contents induced by SNP was markedly
attenuated by CsA. SNP (1 micrometer apprx 1 mM) increased the cGC activity
dose-dependently, and the increase was completely abolished by CsA. CsA
attenuated the SNP-induced cGC activation dose-dependently. The
abolishing effect of CsA-pretreatment on the SNP-induced cGC activation
was not affected by washing the preparation, suggesting that the
inhibition is irreversible. When CsA was added simultaneously with SNP,
cGC activation was not attenuated. 1-(5-isoquinolinylsulfonyl)-2-methyl
piperazine (H-7), a protein kinase C (PKC) inhibitor, decreased
SNP-induced cGC activation and blocked the CsA-attenuation of cGC
activation. These results suggest that CsA directly inhibits cGC
participating in the CsA-induced impairment of vasodilatation, and that
PKC is involved in the inhibitory action of CsA on cGC.