Antimicrobial Activities of Tapocin(r) against Gram-Positive Cocci

Kim, Sang Il; Choi, Su Mi; Lee, Dong Gun; Wie, Seong Heon; Kim, Yang Ree; Yoo, Jin Hong; Shin, Wan Shik; Kang, Moon Won; Kim, Sun Mi; Hur, Jae Kyun; Kang, Jin Han

Abstract

BACKGROUND: Recent hospital environments have changed, and the most common and important causative agents of nosocomial infections are Gram positive organisms, such as staphylococci, streptococci and enterococci. And increasing resistance of such Gram-positive organisms to antimicrobials, including glycopeptides, is causing big problems in hospital infections. Therefore, we confirmed and compared the antimicrobial activities of glycopeptides [Tapocin(r) (CJ Pharmaceutics. Korea), vancomycin, teicoplanin] against clinical isolates of streptococci(PSSP, PNSSP), staphylococci (MSSA, MRSA, MRCNS) and enterococci.
METHODS
Total 666 strains of Gram-positive cocci, collected in five Catholic University affiliated Hospitals were tested by broth microdilution method using Muller-Hinton broth according to the recommendation of NCCLS (National Committee for Clinical Laboratory Standard, USA).
RESULTS
In PSSP (n=46), MIC range of vancomycin was 0.25-0.5 microgram/mL, and that of Tapocin(r) and teicoplanin was equally 0.015-0.06 microgram/mL. MIC90 of vancomycin was 0.5 microgram/mL, and that of Tapocin(r) and teicoplanin was equally 0.06 microgram/mL. In PNSSP (n=123), MIC range of vancomycin was 0.25-0.5 microgram/mL, and MIC range of Tapocin(r) and teicoplanin was equally 0.03-0.06 microgram/mL. The MIC range of vancomycin against MSSA (n=116) was 2-4 microgram/mL, and that of Tapocin(r) and teicoplanin was equally 0.5-8 microgram/mL. MIC90 of vancomycin was 4 microgram/mL, and that of Tapocin(r) and teicoplanin was equally 2 microgram/mL. In MRSA (n=116), MIC range of Tapocin(r) and teicoplanin was equally 0.25-8 microgram /mL, showing broader range distribution than that of vancomycin (2-4 microgram/mL). MIC90 of vancomycin was 4 microgram/mL, and that of Tapocin(r) and teicoplanin was equally 8 microgram/mL. MIC range against MRCNS (n=14) of vancomycin was 0.25-8 microgram/mL, and that of Tapocin(r) and teicoplanin was equally 0.06- 16 microgram/mL. In enterococci (n=123), the MIC range of vancomycin was 0.5-64 microgram/mL, and that of Tapocin(r) and teicoplanin was equally 0.25-64 microgram/mL. MIC90 of all antibiotics was 8 microgram/mL, but 6 E. faecium resistant to all antibiotics were detected.
CONCLUSION
The results of susceptibility tests showed that the against glycopeptides against Gram- positive cocci, which were isolated in our study university hospitals, seem to be largely unaffected. We also confirmed that the antimicrobial activity of Tapocin(r) was equal to that of teicoplanin.