Korean J Prev Med.  2000 Jun;33(2):226-230.

Comparison of Methods for the Detection of Anti-HBs for Hepatitis B Vaccination Program in Korea

• Sohn HS
• Lee JN
• Urm SH
• Lee JT
• Chun JH

Abstract

OBJECTIVES
The purpose of this study was to suggest a proper method for the detection of heaptitis B surface antibody(anti-HBs) in a screening program for hepatitis B vaccination.
METHODS
Sensivitity, specificity and predictive values were compared between Immunochromatographic assay (ICA) and passive hemagglutination(PHA) in 978 subjects(565 males, 413 females, 19-78 years ranging in age, mean 46.5 years old). EIA was used as a standard method for the detection of HBsAb.
RESULTS
Sensitivity in the detection of anti-HBs of PHA and ICA was 88.7% and 94.9%, specificity was 94.3% and 96.6%, negative predictive value was 96.5% and 98.0%, and positive predictive value was 82.3% and 91.3%, respectively. False negative rate(11.3%) of PHA was higher than that(5.1%) of ICA. The higher the titer of anti-HBs in EIA was, the lower the false negative rate was. There was no false negative result in the cases with 101mIU/ml or more in EIA.
CONCLUSION
We suggest that ICA should be the choice of screening method in the detection of anti-HBs in Hepatitis B vaccination program.

Keyword

Anti-HBs; Immunochromatographic assay(ICA); Passive hemagglutination(PHA)

MeSH Terms

Female
Hepatitis B*
Hepatitis*
Humans
Immunochromatography
Korea*
Male
Mass Screening
Sensitivity and Specificity
Vaccination*