J Korean Surg Soc.  2010 Aug;79(2):79-85. 10.4174/jkss.2010.79.2.79.

Correlation between the Expression Reduction of Insulin-like Growth Factor Binding Protein (IGFBP)-3 and PTEN and the Clinicopathological Parameters in Breast Cancer

• Jung SH
• Youn HJ
• Kim MS
• Kim SY
• Hwang PH
• Lee DY

• KMID: 2211976
• DOI: http://doi.org/10.4174/jkss.2010.79.2.79

Abstract

PURPOSE
Insulin-like growth factor binding protein (IGFBP-3) and phosphatase and tensin homolog (PTEN) are tumor-suppressor genes that may be involved in breast tumorigenesis. However, the roles of these genes in the regulation of breast cancer growth or progress are unclear. In this study, we aimed to find any correlation between the reduction of IGFBP-3 or PTEN protein expression in cancer tissues and the clinicopathological parameters in breast cancer.
METHODS
We collected both cancer and adjacent normal tissues from 46 breast cancer patients (from January 1 to December 31, 2006), and checked the IGFBP-3 and PTEN protein levels in cancer and adjacent normal tissues using Western immunoblot. We evaluated the correlation of reduction status of IGFBP-3 and PTEN protein expression with variable clinicopathological parameters.
RESULTS
The frequency of IGFBP-3 and PTEN protein reduction in cancer tissue, compared to adjacent normal tissue, was 63.0% and 34.8%, respectively. And in 87.5% of patients, who showed significant PTEN reduction, IGFBP-3 protein expression was reduced in cancer tissues. In contrast, IGFBP-3 protein reduced in only 50% of patients who didn't show PTEN reduction. However, we did not find any significant correlation between reduction of IGFBP-3 or PTEN expression in cancer tissue and variable clinicopathological parameters.
CONCLUSION
The IGFBP-3 and PTEN genes were expressed in all breast cancer tissues. Nonetheless, we did not find any significant relationship between reduction of IGFBP-3 or PTEN expression and the clinicopathological parameters in this study. Therefore, further studies are needed to document the roles of IGFBP-3 and PTEN genes in breast cancer growth or progress.

Keyword

Breast cancer; IGFBP-3; PTEN; Clinicopathological parameters

MeSH Terms

Blotting, Western
Breast
Breast Neoplasms
Carrier Proteins
Cell Transformation, Neoplastic
Humans
Insulin-Like Growth Factor Binding Protein 3
Microfilament Proteins
PTEN Phosphohydrolase
Carrier Proteins
Insulin-Like Growth Factor Binding Protein 3
Microfilament Proteins
PTEN Phosphohydrolase

Figure

• 

  Fig. 1 Expression of insulin-like growth factor binding protein (IGFBP)-3 in normal and cancer tissues in human breast cancer. Total protein from normal (N) and cancer (C) tissues from 4 individual breast cancer patients (P1 to P4) were isolated, and subjected to Western immunoblot, as described in the Methods section.

• 

  Fig. 2 Expression of phosphatase and tensin homolog (PTEN) protein in normal and cancer tissues in human breast cancer. Total protein from normal (N) and cancer (C) tissues from 4 individual breast cancer patients (P1 to P4) were isolated, and subjected to Western immunoblot.

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