J Genet Med.  2008 Jun;5(1):55-60.

Karyotype analysis of cryopreserved mononuclear cells from cord blood

Affiliations
  • 1Hanyoung Hanmaum Pediatric Clinic, Daegu, Korea.
  • 2Department of Pediatric, Kyungpook National University School of Medicine, Daegu, Korea. kslee@knu.ac.kr
  • 3Department of Pediatric, Chungbuk National University School of Medicine, Cheongju, Korea.

Abstract

PURPOSE
The ability to perform chromosome analysis of cryopreserved cord blood mononuclear cells is important for future retrospective studies. We compared the karyotypes of cryopreserved cells with cells before cryopreservation.
METHODS
One cord blood (CB) sample was obtained from normal healthy volunteer. Karyotype analysis was performed before cryopreservation. After mononuclear cell separation with Ficoll-Hypaque, the mononuclear cells were cryopreserved by programmed controlled-rate freezer and then transferred into the liquid nitrogen (-196 degrees C) for 3 days. After rapid thawing, cytogenetic analysis was performed as the same method for each sample by different conditions. The samples were divided by three groups. The first group was no culture before cryopreservation, the second group was 72 hours culture before cryopreservation, but no 24 hours culture after thawing and the third group was 72 hours culture before cryopreservation and 24 hours culture after thawing.
RESULTS
The chromosome analysis was successful in the second and third groups of CB sample.
CONCLUSION
The successful result from CB samples may suggest the usefulness of long-term cryopreservation for retrospective study in various clinical settings including hematologic malignancies.

Keyword

Karyotype analysis; Cryopreservation; Cord blood mononuclear cell
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