Lab Med Online.  2016 Apr;6(2):111-115. 10.3343/lmo.2016.6.2.111.

Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients

Affiliations
  • 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea. leekcp@yuhs.ac
  • 2Department of Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea.

Abstract

In this study, we report three cases in which two species of the Bacteroides fragilis group, 'Bacteroides nordii' and 'Bacteroides salyersiae', were isolated from peritoneal fluid cultures from post-operative peritonitis patients. The two species of the B. fragilis group were initially misidentified as B. fragilis/Bacteroides stercoris and Bacteroides ovatus by Rapid ID 32A (bioMérieux, France), and finally confirmed as B. nordii and B. salyersiae using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16s rRNA sequencing. For the identification of anaerobes, particularly B. fragilis group organisms, MALDI-TOF MS is a useful method not only because of its concordance with 16S rRNA sequencing results, but also because of its rapidity and simple procedure.

Keyword

Bacteroides fragilis group; Bacteroides nordii; Bacteroides salyersiae; Spectrometry; Mass; Matrix-assisted laser desorption-ionization
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