Yonsei Med J.  2008 Oct;49(5):828-835. 10.3349/ymj.2008.49.5.828.

Expression and Evaluation of Chikungunya Virus E1 and E2 Envelope Proteins for Serodiagnosis of Chikungunya Virus Infection

• Cho B
• Jeon BY
• Kim J
• Noh J
• Kim J
• Park M
• Park S

• KMID: 2158202
• DOI: http://doi.org/10.3349/ymj.2008.49.5.828

Abstract

PURPOSE
Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic tests such as hemagglutination inhibition test (HI test), in-house IgM capture enzyme-linked immunosorbent assays (ELISA), and indirect immunofluorescence test were used for diagnosis of chikungunya fever, which are based on whole virus antigens. MATERIALS AND METHODS: CHIKV E1, and E2 envelope proteins for the CHIKV-specific serodiagnostic reagents for chikungunya fever were expressed in baculovirus expression system. The seroreactivity of recombinant CHIKV E1 and E2 envelope proteins were evaluated using sera panels of patients from Laboratoire Marcel Merieux by indirect IgM capture ELISA. RESULTS: The recombinant CHIKV E1 and E2 envelope protein showed sensitivity of 77.5% and 90%, respectively. The specificities of both CHIKV E1 and E2 envelope proteins were 100%. CONCLUSION: The recombinant CHIKV E1 and E2 envelope proteins could be a useful diagnostic reagent for CHIKV infection.

Keyword

Chikungunya virus; E1 and E2; envelope protein; baculovirus; indirect IgM ELISA

MeSH Terms

Alphavirus Infections/*diagnosis
Animals
Baculoviridae/genetics/metabolism
Cells, Cultured
Chikungunya virus/genetics
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay/methods
Recombinant Proteins/immunology
Sensitivity and Specificity
Serologic Tests/*methods
Viral Envelope Proteins/*immunology