J Korean Med Sci.  2006 Aug;21(4):627-632. 10.3346/jkms.2006.21.4.627.

Granulocyte Antibodies in Korean Neonates with Neutropenia

• Han TH
• Chey MJ
• Han KS

• KMID: 2157812
• DOI: http://doi.org/10.3346/jkms.2006.21.4.627

Abstract

Neonatal alloimmune neutropenia (NAN) is a disease that can cause severe and prolonged neutropenia in neonates. However, no report is available on the incidence of granulocyte antibody in neonates, the target antigen of this antibody, and the estimated incidence of NAN in Korea. Among a total of 856 neonates admitted to a neonatal intensive care unit (NICU) over a five year period, a total of 105 neonates with neutropenia were enrolled in this study. Positive reactions were observed in the sera of six neonates (5.7%, 6/105) by mixed passive hemagglutination assay (MPHA). To confirm the presence of NAN, MPHA and granulocyte antigen typing (HNA-1a, -1b, -2a, -4a, and -5a) were performed on neonatal and maternal blood. To differentiate granulocyte antibody and HLA antibody, MPHA was also performed using HLA antibody adsorbed serum. We confirmed three cases (2.9%, 3/105) of NAN among neonates with neutropenia in which granulocyte antibody specificities (two anti-HNA-1b and one anti-HNA-1a) and fetomaternal granulocyte antigen mismatches were identified. In this study, the estimated incidence of NAN was 0.35% (3/856) among neonates admitted to NICUs in Korea.

Keyword

Neutropenia; Neonatal Alloimmune Neutropenia; Granulocyte Antibodies

MeSH Terms

Polymerase Chain Reaction/methods
Neutropenia/blood/diagnosis/*immunology
Korea
Isoantigens/genetics/immunology
Isoantibodies/*immunology
Intensive Care Units, Neonatal/statistics & numerical data
Infant, Newborn
Humans
Hemagglutination Tests
HLA Antigens/immunology
Granulocytes/*immunology
Genotype
Antibody Specificity/immunology