Mycobiology.  2005 Mar;33(1):7-11. 10.4489/MYCO.2005.33.1.007.

Induction of Sexual Stage and Colony Morphology of Some Isolates of Sclerotium rolfsii Causing Spotted Leaf Rot in Plants

• Pandey MK
• Sarma BK
• Singh UP

• KMID: 2052857
• DOI: http://doi.org/10.4489/MYCO.2005.33.1.007

Abstract

Twenty-two isolates of Sclerotium rolfsii causing spotted leaf rot from Varanasi, India were grown on 6% Cyperus rotundus rhizome meal agar (CRMA) medium for the induction of athelial stage (Athelia rolfsii). Only one isolate obtained from Sphaeranthus indicus formed basidial stage on CRMA medium while the other 21 isolates did not. Basidial stage was also produced in S. indicus isolate at different concentrations (5.5, 6.0 and 6.5% w/v) of CRMA medium. Size of basidia, sterigmata and basidiospores of this isolate was measured. Basidia clavate, hyaline and measured 10~12 x 4~5 microm in size, basidiospores hyaline, unicellular, subglobose to ellipsoid produced on sterigmata and measured 3~5 x 2~4microm in size, sterigmata hyaline and measured 4~5 x 1.5~2 microm in size. The results of the present study revealed wide variation in spotted leaf rot isolates of S. rolfsii. A reddish zone around the colony of S. rolfsii isolate from Vernonia sp. was observed on CRMA medium. HPLC analysis of the zone revealed the presence of gallic and ferulic acid which were also thought to be responsible for reduced mycelial growth of the isolate on CRMA medium.

Keyword

CRMA; HPLC analysis; Phenolic acids; Sclerotium rolfsii; Sexual stage induction

MeSH Terms

Agar
Asteraceae
Chromatography, High Pressure Liquid
Cyperus
Hyalin
India
Meals
Rhizome
Vernonia
Agar