Yonsei Med J.  2008 Aug;49(4):647-654. 10.3349/ymj.2008.49.4.647.

Effectiveness of Different Cleaning Agents against the Colonization of Candida spp and the in Vitro Detection of the Adherence of These Yeast Cells to Denture Acrylic Surfaces

• Nalbant AD
• Kalkanci A
• Filiz B
• Kustimur S

• KMID: 1793202
• DOI: http://doi.org/10.3349/ymj.2008.49.4.647

Abstract

PURPOSE
The aim of this study is to examine the effect Klorhex and Fittydent, which are used as cleaning agents on the adhesion of Candida on the surfaces of acrylic denture and palatal mucosa. In addition, ability of yeasts to adhere to acrylic strips was evaluated after applying these agents in vitro. MATERIALS and METHODS: Each group of 15 patients cleaned their dentures with either Klorhex or with Fittydent. The control group cleaned their dentures with water. RESULTS: It was found that 62.2% of the patients had colonies of Candida species on their palatal mucosa which was reduced to 51.1% after using these cleaning agents. The colonization rate with Candida spp on their dentures was reduces from 82.2% to 68.8% using these cleaning agents. The mean adhesion value of the Candida strains isolated from the acrylic strips were found to be 75cell/strip prior to applying the Klorhex and Fittydent and 37.5cell/strip and 15cell/strip after applying these agents, respectively. CONCLUSION: These results showed that Klorhex and Fittydent have a certain preventive effect on the colonization rate of Candida spp on the surface of these dentures, the palatal mucosa, as well as on the acrylic strips in vitro.

Keyword

Candida; colonization; adhesion; acrylic

MeSH Terms

*Acrylates
Adhesiveness/drug effects
Antifungal Agents/*pharmacology
Candida/*cytology/*drug effects
Cell Adhesion/drug effects
Denture Cleansers/*pharmacology
*Dentures
Mouth Mucosa/drug effects/microbiology
Polyvinyls/pharmacology

Figure

• 

  Fig. 1 Boxplot of the colonization value of the Candida species on the palatal mucosa before and after using the cleaning agents. According to the Non parametric Friedman two-way analysis of variance; Water (i), Klorhex (j); |Ri-Rj| = 12; critical value > 10.641; significance. There was no significant difference between the water and Fittydent; Fittydent and Klorhex.

• 

  Fig. 2 Boxplot of the colonization value of Candida species on the denture surfaces before and after using the cleaning agents. According to the Non parametric Friedman two-way analysis of variance; Water (i), Fittydent (j); |Ri-Rj| = 10.95; critical value > 10.730; significance. There was no significant difference between water and Klorhex; Klorhex and Fittydent.

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