J Vet Sci.  2014 Jun;15(2):273-281. 10.4142/jvs.2014.15.2.273.

Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows

• Rasolomboahanginjatovo HS
• Chorfi Y
• Dupras R
• Mills L
• Lefebvre R

• KMID: 1784649
• DOI: http://doi.org/10.4142/jvs.2014.15.2.273

Abstract

The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at day 7 of the estrous cycle to start the SOV protocol, as well as on the day of embryo recovery (DER). The SOV group produced 7.5 +/- 6.7 oocytes/embryos, of which 3.4 +/- 4.7 were TE. Serum urea and E2 and uterine Glu, CK, LDH, TP, P4 and PGFM in the control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p < 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p < 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE.

Keyword

biochemical parameters; dairy cows; superovulation; transferable embryos

MeSH Terms

Animals
Blood Chemical Analysis/veterinary
Cattle/blood/*embryology/*physiology
Embryo Transfer/veterinary
*Embryonic Development
*Estrous Cycle
Female
Superovulation
Uterus/*chemistry/cytology/*microbiology

Figure

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  Fig. 1 Correlations between serum and uterine PGFM (pg/mL) at the day of embryo recovery (DER) in seven single-ovulated non-lactating dairy cows, control group (A) and at day 7 and DER in 28 superovulated lactating cows, SOV group (B and C).

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  Fig. 2 Correlation between serum and uterine P4 (ng/mL) at day 7 in 28 superovulated lactating cows, SOV group.

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  Fig. 3 Correlation between the number of TE and serum concentration of P4 (ng/mL) at DER in 28 superovulated lactating cows, SOV group.

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  Fig. 4 Correlations between the number of TE and serum and uterine PGFM (pg/mL) at day 7 (r = -0.67, p < 0.001 and r = -0.54, p < 0.005) and at DER (r = -0.57, p < 0.002 and r = -0.48, p < 0.01) in 28 superovulated lactating cows, SOV group.

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