Korean J Ophthalmol.  1995 Dec;9(2):69-78. 10.3341/kjo.1995.9.2.69.

Induction of vitreolysis and vitreous detachment with hyaluronidase and perfluoropropane gas

• Kang SW
• Hyung SM
• Choi MY
• Lee J

• KMID: 1735140
• DOI: http://doi.org/10.3341/kjo.1995.9.2.69

Abstract

To simulate the posterior vitreous detachment (PVD) in the rabbit, 1 IU hyaluronidase and/or 0.2 ml of perfluoropropane gas was intravitreally injected. Ophthalmoscopic, light microscopic examination prepared by cryotechnique, electron microscopic examination, and electroretinogram were done on the 3rd and 28th postoperative days. As a result, the eyes undergone simultaneous intravitreal injection of 1 IU hyaluronidase and 0.2 ml perfluoropropane gas showed membranous structure split from the internal limiting membrane of the superior retina in 3 days after injection. The eyes also demonstrated membranous structure separated from the superior retina after 28 days, simulating vitreous detachment. On the contrary, neither agent alone induced vitreous detachment. No toxic retinal changes associated with simultaneous intravitreal injection of 1 IU hyaluronidase and 0.2 ml perfluoropropane gas were observed. Therefore, with a future support by histologic examination other than cryotechnique and by immunohistochemical analysis, the simultaneous intravitreal injection of perfluoropropane gas and hyaluronidase would be a promising method to induce vitreous detachment in non-vitrectomized eye.

Keyword

vitreous detachment; hyaluronidase; perfluoropropane gas

MeSH Terms

Animals
Drug Combinations
Electroretinography
Eye Diseases/chemically induced/pathology
Fluorocarbons/*toxicity
Hyaluronoglucosaminidase/*toxicity
Injections
Rabbits
Retina/drug effects/physiology
Vitreous Body/*drug effects/pathology
Drug Combinations
Fluorocarbons
Hyaluronoglucosaminidase