Prog Med Phys.  2014 Jun;25(2):116-121. 10.14316/pmp.2014.25.2.116.

The Scattering Beam Measurement of the RBC and the Fabrication of the Micro Cell Biochip

• Byun IS
• Kwon KJ
• Lee JH

• KMID: 1708179
• DOI: http://doi.org/10.14316/pmp.2014.25.2.116

Abstract

Next future, The bio technology will be a rapidly developing. This paper is the scattering beam measurement of the red blood cell (RBC) and the fabrication of the micro cell biochip using the bio micro electro mechanical system (Bio-MEMS) process technology. The Major process method of Bio-MEMS technology was used the buffered oxide etchant (BOE), electro chemical discharge (ECD) and ultraviolet sensitive adhesives (UVSA). All experiments were the 10 times according to the process conditions. The experiment and research are required the ultraviolet expose, the micro fluid current, the cell control and the measurement of the output voltage Vpp (peak to peak) waveform by scattering angles. The transmitting and receiving of the laser beam was used the single mode optical fiber. The principles of the optical properties are as follows. The red blood cells were injected into the micro channel. The single mode optical fiber was inserting in the guide channel. The He-Ne laser beam was focusing in the single mode optical fiber. The transmission He-Ne laser beam is irradiating to the red blood cells. The manufactured guide channel consists of the four inputs and the four outputs. The red blood cell was allowed with the cylinder pump. The output voltage Vpp waveform of the scattering beam was measured with a photo detector. The receiving angle of the output optical fiber is 0degrees, 5degrees, 10degrees, 15degrees. The magnitude of the output voltage Vpp waveform was measured in the decrease according to increase of the reception angles. The difference of the output voltage Vpp waveform is due differences of the light transmittance of the red blood cells.

Keyword

Red blood cell (RBC); Bio micro electro mechanical system (Bio-MEMS); Buffered oxide etxhant (BOE); Electro chemical discharge (ECD); Ultra violet sensitive adhesives (UVSA)

MeSH Terms

Adhesives
Erythrocytes
Optical Fibers
Adhesives

Figure

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  Fig. 1. Mask pattern by auto CAD program.

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  Fig. 2. Channel pattern for red blood cells focusing.

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  Fig. 3. Diameter etching of optical fiber cladding.

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  Fig. 4. Spark discharge of needle end point in solution.

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  Fig. 5. Roughness condition of glass hole etching.

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  Fig. 6. Bonding of optic fiber guide channel.

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  Fig. 7. Completed biochip (a) and laser beam in optical fiber (b).

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  Fig. 8. Sheath current of cell focusing channel.

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  Fig. 9. Voltage output wave of red blood cells by angles.

Reference

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