J Vet Sci.  2013 Sep;14(3):315-321. 10.4142/jvs.2013.14.3.315.

Development of in vitro produced porcine embryos according to serum types as macromolecule

• Son J
• Malaweera
• Lee E
• Shin S
• Cho J

• KMID: 1705562
• DOI: http://doi.org/10.4142/jvs.2013.14.3.315

Abstract

This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS), different types of PS [newborn pig serum, prepubertal gilt serum (PGS), estrus sow serum, and pregnancy sow serum] were used to supplement IVM media with or without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then compared. The maturation rates of the PGS group was significantly higher when GTH was not added. Additionally, during development of PA embryos without GTH, the PGS group showed significantly higher cleavage and blastocyst formation rates. Moreover, the cleavage rates of IVF embryos were significantly higher in the PGS group, with no significant differences in the blastocyst formation. However, when GTH was supplemented into the IVM media, there were no significant differences among the four groups in the cleavage rates, development rates of the blastocyst, and cell number of the blastocyst after PA and IVF. In conclusion, PGS is an efficient macromolecule in porcine IVM, and GTH supplementation of the IVM media is beneficial when PS is used as macromolecule, regardless of its origin.

Keyword

gonadotropin; in vitro fertilization parthenogenesis; porcine; serum

MeSH Terms

Animals
Blastocyst/*drug effects
Embryo, Mammalian/drug effects/*embryology/physiology/ultrastructure
Fertilization in Vitro/veterinary
Gonadotropins/administration & dosage/*metabolism
In Vitro Oocyte Maturation Techniques/*methods/veterinary
Parthenogenesis/*drug effects
Sus scrofa/*embryology
Gonadotropins

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