Korean J Nucl Med.  2000 Jun;34(3):252-259.

Comparison of Radiation Adaptive Responses in Peripheral Lymphocytes of Patients Undergoing Tc-99m MDP and Tc-99m DTPA Scintigraphies

• Rom HS
• Li MH
• Min JJ
• Kwon AS
• Kim JY

Abstract

PURPOSE: The purpose of this study was to compare the radiation adaptive response (RAR) in peripheral lymphocytes (PL) of patients induced by Tc.-99m MDP and Tc-99m DTPA scintigraphies.
MATERIALS AND METHODS
Lymphocytes from 45 patients (25 males, 20 females, mean age 44+/-18 years) were collected before and after scintigraphies using 740 MBq Tc-99m MDP (n=22) or Tc-99m L)TPA (n=23). Lympho-cytes from 20 controls (12 males, 8 females, mean age 43+/-7 years) were also callected. They were exposed to challenge dose of 2 Gy gamma-rays using a Cs-137 cell irradiator, Number of ring-form (R) and dicentric (D) chromosomes was counted under the light microscope. From them a representative score, Ydr, was calculated as Ydr=(D+R)/cells. Adaptation index (AI) was defined as difference of Ydr between unconditioned and conditioned lymphocytes. Ydr was also measured after an administration of cyclohexi-mide (CHM), a protein synthesis inhibitor, before challenge dose.
RESULTS
RAR was induced in both groups of patients. CHM abolished the adaptive response in both groups. AI of Tc-99m MDP group was significantly higher than that of Tc-99m DTPA group.
CONCLUSION
Tc-99m MDP induced RAR was more prominent than those induced by Tc-99m DTPA.

Keyword

Radiation adaptive response; Peripheral lymphocyte; Chromosomal aberration; Tc-99m methylene diphosphonate (MDP); Tc-99m diethylenetriamine pentaaeetric acid (DTPA)

MeSH Terms

Chromosome Aberrations
Female
Humans
Lymphocytes*
Male
Pentetic Acid*
Technetium Tc 99m Medronate*
Pentetic Acid
Technetium Tc 99m Medronate