J Korean Soc Virol.  1997 Dec;27(2):137-141.

The Evaluation of Immunochromatographic Assay kit for Rapid Detection of Hepatitis B Surface Antigen

• Shin HS
• Kim YB
• Shin JW
• Kim CK
• Lee WS
• Kim HK
• Shin KS

Abstract

We evaluated Immunochromatographic assay kit to screen HBsAg in human serum. When the reference HBsAg was applyed to ICA, HA and EIA kits, the limit of detection for HBsAg were found out to be 4, 2 and 0.25 ng/ml respectively. But ICA kit required 5 minutes to read the result whereas HA and EIA kit more than one hour. The sensitivity was 97% (29 of 30 samples) and the specificity 100% (45 samples) compared with conventional EIA. The ICA kit needs no instrument or machine to perform the test contrary to the conventional methods. Therefore, this rapid and sensitive ICA kit can be used for HBsAg-screening, especially in the emergency room and in the scene of the accident.

MeSH Terms

Emergency Service, Hospital
Hepatitis B Surface Antigens*
Hepatitis B*
Hepatitis*
Humans
Immunochromatography*
Limit of Detection
Sensitivity and Specificity
Hepatitis B Surface Antigens