Korean J Parasitol.  2013 Feb;51(1):129-132. 10.3347/kjp.2013.51.1.129.

Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle

Affiliations
  • 1Islamic Azad University of Jahrom, Jahrom, Iran.
  • 2Razi Vaccine and Serum Research Institute, Shiraz, Iran. namavari@yahoo.com

Abstract

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.

Keyword

Neospora caninum; Cattle; Dot dye immunoassay; Colloidal dye

MeSH Terms

Animals
Antibodies, Protozoan/*blood
Cattle
Cattle Diseases/*diagnosis/parasitology
Coccidiosis/diagnosis/parasitology/*veterinary
Diagnostic Tests, Routine/*methods
Female
Immunoassay/methods
Neospora/*immunology
Staining and Labeling/methods
Veterinary Medicine/*methods
Antibodies, Protozoan
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