J Vet Sci.  2013 Mar;14(1):99-102. 10.4142/jvs.2013.14.1.99.

Genetic IS901 RFLP diversity among Mycobacterium avium subsp. avium isolates from four pheasant flocks

• Moravkova
• Lamka J
• Slany M
• Pavlik I

• KMID: 1482820
• DOI: http://doi.org/10.4142/jvs.2013.14.1.99

Abstract

IS901 RFLP analysis of 36 Mycobacterium avium subsp. avium (MAA) isolates from 15 pheasants (Phasianus colchicus) and two goshawks (Accipiter gentilis) from four pheasant farms was performed. Using this method, six different IS901 RFLP types (E, F, G, M, Q, and V) were identified. The distribution of IS901 RFLP profiles was tightly linked to individual flocks. Matching IS901 RFLP profiles observed in the present study indicate MAA transmission between pheasants and goshawks in the same locality. In two flocks, different pheasants within a flock as well as in various organs of five individual pheasants were found to have two distinct IS901 RFLP profiles.

Keyword

avian tuberculosis; captive birds; genotyping; MAC; zoonosis

MeSH Terms

Animals
Bone Marrow/microbiology
*Galliformes
Intestines/microbiology
Liver/microbiology
Mycobacterium avium/*genetics
*Polymorphism, Genetic
*Polymorphism, Restriction Fragment Length
Poultry Diseases/*microbiology
Spleen/microbiology
Tuberculosis, Avian/*microbiology

Figure

• 

  Fig. 1 Six IS901 RFLP profiles of Mycobacterium avium subsp. avium isolates after digestion with restriction endonuclease PvuII. Band sizes of a 1-kbp ladder are shown on the left of the patterns. Arrowheads indicate differences between IS901 RFLP profiles in an individual flock. Flock A: IS901 RFLP profiles G and Q, flock B: IS901 RFLP profiles F and M.

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