Lab Anim Res.  2011 Dec;27(4):339-342. 10.5625/lar.2011.27.4.339.

Subcellular localization of the transmembrane inner ear (Tmie) protein in a stable Tmie-expressing cell line

  • 1Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Korea.
  • 2Institute of Natural Medicine, Hallym University, Chuncheon, Korea.
  • 3Department of Microbiology, College of Medicine, Hallym University, Chuncheon, Korea.


Mutations in the transmembrane inner ear (Tmie) gene, which encodes the Tmie protein, have been attributed to deafness autosomal recessive 6 (DFNB6), an autosomal nonsyndromic recessive hearing loss disorder. Although the Tmie gene was identified a few years ago, little is known about subcellular localization of the Tmie protein. In order to address this, we developed a stable cell line expressing Tmie protein. The expression of Myc-tagged Tmie protein was confirmed by Western blot analysis using an anti-Myc antibody and localization of the Tmie protein was confirmed by immunostaining, using the anti-Myc antibody as well as the anti-tmie antibody. Our study demonstrates that the Tmie protein is localized mostly in the cellular membrane and to a lesser extent in cytoplasm. These results suggest that our Tmie expressing stable cell line provides a suitable in vitro model to explore Tmie synthesis and functions.


Transmembrane inner ear protein; subcellular localization; hearing loss disorder; HEK293
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