Korean J Ophthalmol.  2012 Feb;26(1):32-38. 10.3341/kjo.2012.26.1.32.

Retinal Nerve Fiber Layer Measurement Variability with Spectral Domain Optical Coherence Tomography

• Hong JT
• Sung KR
• Cho JW
• Yun SC
• Kang SY
• Kook MS

• KMID: 1120179
• DOI: http://doi.org/10.3341/kjo.2012.26.1.32

Abstract

PURPOSE
To evaluate the effect of the scanning laser ophthalmoscope (SLO) guided re-test mode on short- and long-term measurement variability of peripapillary retinal nerve fiber layer (RNFL) thickness obtained by spectral domain-SLO optical coherence tomography (SD-SLO/OCT).
METHODS
Seventy five healthy eyes were scanned 3 times per day (intra-session variability) by both the SLO guided re-test mode and the independent mode of SD-SLO/OCT. Subjects were scanned 3 times by both modes at visits within a 2-week interval (inter-session variability). For testing longitudinal variability, 3 separate exams were performed over 6 months by both modes. The coefficient of variation (CV), reproducibility coefficient (RC) and intraclass correlation coefficient of RNFL thickness were compared between the two modes.
RESULTS
The intra-session RC and CV ranged from 5.4 to 12.9 microns and 1.76% to 5.72% when measured by independent mode and 5.4 to 12.5 microns and 1.75% to 5.58% by re-test mode, respectively. The inter-session RC and CV ranged from 5.8 to 13.3 microns and 1.89% to 5.78% by independent mode and 5.8 to 12.7 microns and 1.90% to 5.54% by re-test mode, respectively. Intra-session and inter-session variability measurements were not significantly different between the two modes. The longitudinal RC and CV ranged from 8.5 to 19.2 microns and 2.79% to 7.08% by independent mode and 7.5 to 14.4 microns and 2.33% to 6.22% by re-test mode, respectively. Longitudinal measurement variability was significantly lower when measured by the re-test mode compared to the independent mode (average, p = 0.011).
CONCLUSIONS
The SLO guided re-test mode for RNFL thickness measurement in SD-SLO/OCT employing a tracking system improved long-term reproducibility by reducing variability induced by inconsistent scan circle placement.

Keyword

Optical coherence tomography; Reproducibility; Retinal nerve fiber layer; Tracking system

MeSH Terms

Adult
Algorithms
Anatomy, Cross-Sectional
Female
Humans
Male
Nerve Fibers
Ophthalmoscopes
Reference Values
Reproducibility of Results
Retinal Ganglion Cells/*cytology
Tomography, Optical Coherence/*methods

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