Korean J Lab Med.  2009 Jun;29(3):179-184. 10.3343/kjlm.2009.29.3.179.

Complete Blood Count Reference Values of Donated Cord Blood from Korean Neonates

• Lee HR
• Shin S
• Yoon JH
• Kim BJ
• Hwang KR
• Kim JJ
• Roh EY

• KMID: 1096967
• DOI: http://doi.org/10.3343/kjlm.2009.29.3.179

Abstract

BACKGROUND: In the public cord blood (CB) banks, only safe CB units with adequate cell doses are processed and stored. Complete blood count (CBC) of CB is crucial for estimating total nucleated cells (TNC) and screening suitable CB units without hematologic abnormalities. We analyzed CBC parameters of the donated CB from healthy Korean neonates to establish CBC reference values.
METHODS
A total of 2,129 Korean CB units, donated and processed during the period from August 2007 to December 2007, were enrolled. We measured hemoglobin (Hb), white blood cell (WBC) count, differential count of WBC, platelets and nucleated red blood cell (nRBC) count by XE-2100 automated hematology analyzer (Sysmex, Japan), and estimated reference value of each parameter by using parametric (Mean+/-2SD) and/or non-parametric methods (2.5-97.5 percentile). And also, we compared the result of each parameter in relation to sex of neonates and delivery method.
RESULTS
Because the differences of CBC values among different subgroups were not remarkable, we established the reference intervals as follows without subgroup division: Hb, 9.0-14.4 g/dL; WBC count, 5.6-18.5x103/microL; differential count of WBC (neutrophils, 40.8-72.4%; lymphocytes, 17.2-46.7%; monocytes, 4.9-12.8%; eosinophils, 0.7-7.0%; basophils, 0.0-1.6%); platelet, 130-287x103/microL; nRBCs, 0.0-13.1/100 WBC.
CONCLUSIONS
We established cord blood CBC reference values of healthy Korean neonates using a large-scale CB units. The established CBC reference values from our study will be useful as basic data for CBC interpretation and assessment of transplant suitability of donated CB.

Keyword

Donated cord blood; Reference value; CBC

MeSH Terms

Blood Cell Count/*standards
Female
Fetal Blood/*cytology
Humans
Infant, Newborn
Korea
Male
Reference Values

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