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Consideration of Appropriateness of Application of Immunoblot Assay as a Reentry Test for HCV or HIV Screening Reactive Donors

Kang JW, Youn KW, Seo JH, Seo YI, Park S

BACKGROUND: Currently, serological assay, immunoblotting, and nucleic acid amplification test (NAT) are required as reentry tests for deferred donors with hepatitis C virus (HCV) or human immunodeficiency virus (HIV) screening...
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Immunoblot Patterns of Taenia asiatica Taeniasis

Jeon HK, Eom KS

Differential diagnosis of Taenia asiatica infection from other human taeniases by serology has been tested. An enzyme-linked immunoelectrotransfer blot (EITB) was applied to subjected human sera and tapeworm materials. Thirty-eight...
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Differentiation of Entamoeba histolytica and Entamoeba dispar in cyst-passers by immunoblot

Lee MJ, Hong ST

Differentiation of invasive strains of Entamoeba histolytica according to their pathogenicity has been a topic of long debate, but now the pathogenic species only is regarded as E. histolytica while...
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Detection Of Antibodies To Ena In The Systemic Lupus Erythematosus By Immunoblotting: A Cornparision Of Irnmunoblotting And Double Immunodiffusion

Park SH, Lee SH, Cho CS, Kim HY

  • KMID: 2025496
  • J Korean Rheum Assoc.
  • 1995 Jan;2(1):34-41.
OBJECTIVE: Systemic Lupus Erythematosus(SLE) is characterized by various autoantibodies to a variety of nuclear antigens. Certain extractable nuclear antigens(ENA) have been served as extremly useful aids in differentiation of clinical...
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Antibody Response of Infants to Helicobacter pylori Infection

Kim EA, Kim YO, Lim JY, Jung YS, Park CH, Woo HO, Youn HS, Ko GH, Baik SC, Lee WK, Cho MJ, Rhee KH

  • KMID: 1857604
  • Korean J Gastroenterol.
  • 2000 Jun;35(6):704-715.
BACKGROUND/AIMS: The early stage of Helicobacter pylori (H. pylori) infection may show only anti-H. pylori IgM antibody response in young infants. We tried to find out the antigens of H....
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Skin ulcer and immunoblot patterns by inoculation sites in BALB/c mice infected with Leishmania major

Lee M, Lee JK, Kim SB, Lee SH, Hong ST

The skin ulcer in Leishmania major infection is known to be variable according to the inoculation sites even in a susceptible host. The present study traced the immunoblot patterns by...
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Application of bispecific antibody against antigen and hapten for immunodetection and immunopurification

Kim H, Park S, Lee HK, Chung J

  • KMID: 2154262
  • Exp Mol Med.
  • 2013 Sep;45(9):e43.
We present a bispecific antibody that recognizes an antigen and a hapten and can be applied to various biological assays, including immunoblotting and immunoprecipitation. In immunoblot analysis of serum, an...
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Immunoblot findings of calcareous corpuscles binding proteins in cyst fluid of Taenia solium metacestodes

Yang HJ

After collecting calcareous corpuscles from plerocercoid of Spirometra mansoni (sparganum), we evaluated the antigenic values of calcareous corpuscles binding proteins obtained from the cyst fluid of Taenia solium metacestodes. Immunoblot...
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Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites

Lee EG, Kim JH, Shin YS, Shin GW, Kim YH, Kim GS, Kim DY, Jung TS, Suh MD

  • KMID: 1120070
  • J Vet Sci.
  • 2004 Jun;5(2):139-145.
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the...
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Protein composition and antigenicity of the tegument from Paragonimus westermani

Kim SI, Cho SY

To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component...
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Serological observation of Toxoplasma gondii prevalence in Apodemus agrarius, a dominant species of field rodents in Korea

Jeon SH, Yong TS

Field rodents involved in ecological food chains and which are the prey of carnivores in the natural environment may serve as reservoir hosts for Toxoplasma gondii infection in humans, however,...
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Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes

Shin YS, Lee EG, Jung TS

  • KMID: 1114858
  • J Vet Sci.
  • 2005 Jun;6(2):157-160.
The present study was attempted to compare the Neospora caninum (N. caninum) antigenic bands recognized by different bovine immunoglobulin classes. A total 10, 5, 2, and 6 antigenic bands were...
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Helicobacter pylori Antigens Inducing Early Immune Response in Infants

Seo JH, Youn JH, Kim EA, Jun JS, Park JS, Yeom JS, Lim JY, Woo HO, Youn HS, Ko GH, Park JS, Baik SC, Lee WK, Cho MJ, Rhee KH

To identify the Helicobacter pylori antigens operating during early infection in sera from infected infants using proteomics and immunoblot analysis. Two-dimensional (2D) large and small gel electrophoresis was performed using...
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Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand

Kitkhuandee A, Munkong W, Sawanyawisuth K, Janwan P, Maleewong , Intapan PM

Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the...
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IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis

Chung YB, Kong Y, Yang HJ, Cho SY

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially...
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Comparative Study of Anti-HCV by New HCV EIA, Immunoblotting and RT-PCR in Korean Blood Donors

Eun SJ, Suh JS, Lee WK, Kim JS, Kim IS, Kim JG, Song DH

  • KMID: 2089980
  • Korean J Blood Transfus.
  • 1994 May;5(1):9-16.
A newly developed third generation enzyme immunoassay(Lucky HCD 3.0 EIA) for hepatitis C virus(HCV) antibodies was added with the envelope(E1E2)/NS4 fusion proteins and expanded NS5 proteins as well as the...
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Changes of IgM and IgG antibody levels in experimental rabbit anisakiasis as observed by ELISA and SDS-PAGE/immunoblot

Yang HJ, Cho YJ, Paik YH

Antibody changes in experimental anisakiasis were observed in 10 rabbits which were infected each with 10 Anisakis simplex larvae. The sera were collected before and on the 6th to the...
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Evaluation of Anti-dsDNA Antibody Tests: Crithidia luciliae Immunofluorescence Test, Immunoblot, Enzyme-linked Immunosorbent Assay, Chemiluminescence Immunoassay

Yang JY, Oh EJ, Kim Y, Park YJ

BACKGROUND: Anti-double stranded DNA antibody (anti-dsDNA) test is useful for the diagnosis and monitoring of systemic lupus erythematosus (SLE). Although several methods are available, none of them is completely satisfactory...
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Antibody changes in paragonimiasis patients after praziquantel treatment as observed by ELISA and immunoblot

Cho SY, Kim SI, Kang SY, Kong Y, Han SK, Shim YS, Han YC

To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels...
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Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)

Shin G, Lee H, Palaksha K, Kim Y, Lee E, Shin Y, Lee E, Park K, Jung T

The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG...
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