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Immunoradiometric assay of human proinsulin and partially processed proinsulin with use of monoclonal antibody and streptavidin-biotin labeling

Kim JQ, Cho HI, Kim SI, Lee HK, Hales CN

The sensitive and specific immunoradiometric assay is described for human proinsulin and its intermediate peptides (65-66 split and 32-33 split proinsulin). We developed a monoclonal antibody-based two-site immunoradiometric assay with...
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Immunohistochemical Expression of Glutathione S-Transferase pai in Hepatocellulae Carcinoma and Cholangiocarcinoma

Choi SR, Han SY, Son YG, Lee JH, Park HS, Roh MH, Choi HJ, Huh GY

  • KMID: 2143755
  • Korean J Gastroenterol.
  • 1998 Jan;31(1):58-63.
BACKGROUND AND AIMS: It has been known that glutathione S-transferase n (GST-n ) is overexpressed in chemically induced precancerous or cancerous lesions of the hepatocyte in animal models. Unlike most...
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Localization of Intracellular IVIonoclonal Antibody Specific for IVlycobacteria in Experimentally Induced Pulmonary Tuberculosis

Cho SN, Kim DS, Shin DH, Shin JS, Kim SJ, Park CY, Lee JD, Chung SH

  • KMID: 1900012
  • J Korean Radiol Soc.
  • 1994 Aug;31(2):267-271.
PURPOSE: To determine whether intracellular localization of intravenously injected infection specific MoAb can be localized in the infected cells, immunohistochemical staining was obtained in animals infected with Mycobacterium tuberculosis. MATERIALS AND...
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Immunohistochemical Studies of bc1-2 Protein in a Skin Tumor of Neuroectodermal Origin

Seo JJ, Won YH, Kim SJ, Lee SC, Chun IK

  • KMID: 2085948
  • Korean J Dermatol.
  • 1997 Aug;35(4):667-673.
BACKGROUND: Protooncogene, bcl-2 is known to inhibit, apoptosis induced by various stimuli. Its expression has been reported in various fetal and adult tissues, and also in tumors of neural origin....
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Radioimmunoassay for Determination of Serum Macrophage Migration Inhibitory Factor

Lee TS, Shin SH, Song JI, Woo KS, Chung WS, Choi CW, Lim SM

  • KMID: 1990854
  • Korean J Nucl Med.
  • 2004 Dec;38(6):532-539.
PURPOSE: There has been a renewal of interest in Macrophage migration inhibitory factor (MIF), especially correlation in pathogenesis of sepsis by many infectious diseases and in regulation of host inflammatory...
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Development of an Immuno-PCR Protocol for Detection of a Small Amount of Antigen

Jeon CH, Koh EH

  • KMID: 2239000
  • Korean J Lab Med.
  • 2005 Feb;25(1):66-70.
BACKGROUND: Immuno-PCR has been known as a highly sensitive and specific method, yet no standardized protocol is available. We analyzed each step of immuno-PCR to develop a reliable standardized method. METHODS:...
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Evaluation of ES-300 for the Detection of Anti-HCV Antibody

Park JW, Song JH, Park HS, Kang HJ, Lee KM

  • KMID: 2225582
  • Korean J Clin Pathol.
  • 1997 Apr;17(2):313-320.
BACKGROUND: A fully automated enzyme-immunoassay (EIA) analyzer, Enzymun System, ES-300 (Boehringer Mannheim, Germany) uses streptavidin technology and performs single test or panels of up to 12 tests per run. We...
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Comparison in Expression of CD 1 , HLA - DR and ICAM - 1 of Follicular Keratinocytes from a Lesion and a Non - Lesional Scalp of Alopecia Areata

Lee WS, Ahn SK, Lee SH

  • KMID: 2230790
  • Korean J Dermatol.
  • 1995 Oct;33(5):873-879.
BACKGROUND: Primary targit injured from the immunologic mechanism of alopecia areata is not definitely confirmed although alopecia areata is regarded as a disease occuring from certain im munologic process. Recently,...
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Comparison Study between Immunohistochemical Staining and Enzyme Linked Immunosorbent Assay in Metalloproteinases and Tissue inhibitor of Metalloproteinases in Virus induced Myocarditis

Hong YM, Kim SS

  • KMID: 2207692
  • J Korean Pediatr Soc.
  • 1999 Nov;42(11):1524-1534.
PURPOSE: Most tissue disruption of extracellular matrix is mediated by extracellular proteinases. Matrix metalloproteinases(MMP) and tissue inhibitor of matrix metalloproteinases(TIMP) are associated with wound healing and repair. There has been...
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An Immunohistochemical Stain in Paget's Disease

Lee HT, Chang SH, Yoon TY

  • KMID: 2303420
  • Korean J Dermatol.
  • 1998 Oct;36(5):796-803.
BACKGROUND: Generally, there are few problems in the diagnosis of Paget's disease(PD) using the H&E stain. However, the differentiation of PD from the clonal type of Bowens disease and superficial...
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Immunohistochemical and Ultrastructural Observation on Small Round Cell Tumors

Suh CH, Yang JY, Lim SC, Kim YL

  • KMID: 2037297
  • Korean J Pathol.
  • 1997 Nov;31(11):1200-1213.
Small round cell tumors such as neuroblastoma, rhabdomyosarcoma, Ewing's tumor, malignant lymphoma and small cell carcinoma are often confused clinically and histologically. To clarify the similarites and differences and to...
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Distinct Expressions of TGF-a among Chronic Hepatitis, Liver Cirrhosis, and Hepatocellular Carcinoma

Yoo BM, Park SS, Lee DH, Lee JD

  • KMID: 2083030
  • Korean J Hepatol.
  • 1997 Dec;3(4):316-328.
BACKGROUND/AIMS: Transforming growth factor-a(TGF-a) is a polypeptide cytokine related to cell proliferation and transformation. TGF-a binds to EGF receptor and stimulating DNA synthesis in liver cell. The hepatitis B virus (HBV) by itself...
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Expression of Vascular Endothelial Growth Factor Protein in Astrocytic Tumors

Park SH, Chang IB, Kim CH, Cho YJ, Cho BM, Shin DI, Oh SM, Kim DW, Nam ES

  • KMID: 2067658
  • J Korean Neurosurg Soc.
  • 2001 Jun;30(6):683-687.
OBJECTIVE: Angiogenesis, the proliferation of capillary endothelial cells, is a vital component in the development, progression, and metastasis of many human tumors. Vascular endothelial growth factor(VEGF) is an endothelial cell-specific...
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Production of Human Fab Monoclonal Antibody to Surface Protein, preS1, of Hepatitis B Virus using Antibody Phage Display Library

Kim HS, Choi IH, Kim IJ, Chung JH, Park SK

  • KMID: 1548380
  • Korean J Immunol.
  • 1997 Dec;19(4):549-558.
Human monoclonal antibodies have considerable potential in the prophylaxis and treatment of viral disease. By cloning human Ig gene segments from the B cells of volunteer into pComb3 phagemid vector,...
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An immunohistochemical study on the expression of growth factor in periodontal tissue during the experimental movement of rat incisors

Lee JH, Kim SC, Kook YA

  • KMID: 2271559
  • Korean J Orthod.
  • 1995 Feb;25(1):31-42.
This study was designed to evaluate the expression of growth factor in periodontal tissue during the experimental movement of rat incisors by LSAB(Labelled streptavidine Biotin) immunohistochemical staining for EGF(Epidermal growth...
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The Prognostic Significnce of p53 Protein Overexpression in Gastric Carcinoma

Rhee JK

  • KMID: 1939736
  • J Korean Surg Soc.
  • 1997 May;52(5):671-678.
To evaluate prognostic significance of p53 gene mutation and p53 protein overexpression, 83 cases of primary gastric carcinoma (stage 1b, II, and III) were analyzed. Immunohistochemical assay using labeled streptavidine...
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Enzyme-linked immunosorbent assay for detecting anti-pertussis toxin antibody in mouse

Choi GS, Huh DH, Han SB, Ahn DH, Kang KR, Kim JA, Choi BM, Kim HR, Kang JH

PURPOSE: Although the DTaP and Tdap vaccines used to prevent pertussis have been used for a long time, there is no standard method for measuring pertussis antigens. Therefore, this preliminary...
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The expression of heat shock protein in the experimental tooth movement in rats

Yoo DW, Kim EC, Kim SC

  • KMID: 2271881
  • Korean J Orthod.
  • 2001 Apr;31(2):249-259.
This study was designed to evaluate the expression of heat shock protein in tooth and surrounding tissue during the experimental movement of rat incisors, by LSAB(labelled streptavidine biotin) immunohistochemical...
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The expression of type I collagen in periodontal tissue during the experimental movement of rat incisors

Kim SC, Jeon IS

  • KMID: 2271589
  • Korean J Orthod.
  • 1996 Aug;26(4):455-466.
This study was designed to evaluate the expression of type I collagen in periodontal tissue during the experimental movement of rat incisors. Twenty-one Sprague-Dawley rats were divided into a control...
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The expression of osteonectin and osteocalcin in the experimental tooth movement in rat

Bae SR, Kim SC

  • KMID: 2271800
  • Korean J Orthod.
  • 1998 Oct;28(5):699-716.
This study was designed to evaluate the expression of non-collagenous protein in periodontal tissue during the experimental movement of rat incisors, by LSAB(labelled streptavidine biotin) mmunohistochemical staining for osteonectin and...
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