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Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy

Yoon WB, Kim H, Kim KG, Choi Y, Chang HJ, Sohn DK

OBJECTIVES: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue...
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Cytokinetic pattern in the thoracic spinal cord of chick embryos (incubation day 5-13) using PCNA staining and TUNEL method

Wang KC, Park IS, Chi JG, Lee MS, Lee YJ, Cho BK

For the cytokinetic studies using spinal cords of chick embryos, chronological patterns of cell proliferation and programmed cell death (apoptosis) should be known. Information in the early stages of...
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Mucin histochemistry by paradoxical concanavalin A staining in early gastric carcinomas

Cho KJ, Myong HH, Jang JJ

Phenotypic expression of tumor cells was investigated in 33 early gastric carcinomas by mucin histochemistry using paradoxical concanavalin A staining. This staining method had been developed to differentiate 3 classes...
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In vitro cytotoxicity of Acanthamoeba spp. isolated from contact lens containers in Korea by crystal violet staining and LDH release assay

Shin HJ, Cho MS, Jung SY, Kim HI, Im KI

In order to observe the cytotoxicity of Acanthamoeba spp., which were isolated from contact lens containers as ethiological agents for the probable amoebic keratitis in Korea, the crystal violet staining...
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Ultrastructural Localization of Cryptosporidium parvum Antigen Using Human Patients Sera

Lee JG, Han ET, Park WY, Yu JR

The antigen location of Cryptosporidium parvum, which stimulates antibody formation in humans and animals, was investigated using infected human sera. Immuno-electron microscopy revealed that antigenicity-inducing humoral immunity was located at...
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An Autoradiographic Study on Metabolism of Serotonin in Cerebral Nerve Cells Using 5-Hydroxytryptophan-C

Choi KD, Ahn SB, Oh YK, Rhim KS, Moon BY

In order to demonstrate autoradiographically the sites of serotonin metabolism in the brain, DL 5-HTP and DL 5-HTP-C14 were intraperitoneally administered to healthy adult mice. In order to distinguish histochemically...
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Peritubular Capillary C4d Deposition in Chronic Allograft Dysfunction

Jeong HJ, Kim SH, Kim YS, Kim SI, Kim MS, Park K

Peritubular capillary (PTC) C4d staining represents a marker for acute humoral rejection, however, the impact of positive staining on chronic allograft dysfunction has received little attention. Ninety-three renal allograft biopsies...
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Nucleolar organizer regions of lymphomas in Korea

Kim I, Park SH, Paik SY

Nucleolar organizer regions (NORs) are loops of DNA which occur in the nucleoli of cells and which possess ribosomal RNA (rRNA) genes. The numbers and/or configurations of NORs have...
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Immunohistochemical study on antigenic phenotype of Langerhans cell histiocytosis

Yang WI

Immunohistochemical study on 26 cases of Langerhans cell histiocytosis (LCH) using several leukocyte antibodies in addition to traditionally used markers (S-100 protein and peanut agglutinin) revealed that the proliferating cells...
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Immunohistochemical analysis of p53 protein expression in benign and malignant skin tumors using a panel of anti-p53 antibodies

Ro YS, Kim JH

The expression of p53 in a variety of benign and malignant skin lesions has been first assessed in frozen sections and then compared with the results obtained in corresponding paraffin-embedded...
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Prevalence of the Hepatitis B Surface Antigen in Various Liver Diseases among Koreans Demonstrated by Orcein Staining of 614 Biopsies

Ro JY, Park CI, Lee YB

To evaluate prevalence of HBsAg in the liver tissue of various liver diseases among Koreans, a total of 614 cases of liver biopsies; hepatitis (104), cirrhosis (99), hepatocellular carcinoma (78),...
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Neuroprotective Effect of Ginseng Total Saponins in Experimental Traumatic Brain Injury

Ji YC, Kim YB, Park SW, Hwang SN, Min BK, Hong HJ, Kwon JT, Suk JS

In the present study, we investigated whether ginseng total saponins (GTSs) protect hippocampal neurons after experimental traumatic brain injury (TBI) in rats. A moderate-grade TBI was made with the aid...
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Effects of chlorinated aliphatic hydrocarbons on the fidelity of cell division in human CYP2E1 expressing cells

Kim A, Parry EM, Parry JM

  • KMID: 1093602
  • Exp Mol Med.
  • 2002 Mar;34(1):83-89.
Chlorinated organic chemicals are widely used in industry and are present in the environment. Five chlorinated aliphatic hydrocarbons, namely 1-2-dichloroethane, 1,1,2-trichloroethane, trichloroethylene, 2,3-dichlorobutane and 1-chlorohexane were investigated to determine their...
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Secondary cutaneous amyloidosis in disseminated superficial porokeratosis: a case report

Kim JH, Yim HE, Kang WH

Disseminated superficial porokeratosis (DSP) is a rare cause of secondary cutaneous amyloidosis. An 83-year-old male patient showed an increase in both size and number of DSP lesions after contracting pulmonary...
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Computed Tomography Guided Percutaneous Injection of a Mixture of Lipiodol and Methylene Blue in Rabbit Lungs: Evaluation of Localization Ability for Video-Assisted Thoracoscopic Surgery

Jin KN, Lee KW, Kim TJ, Song YS, Kim DI

Preoperative localization is necessary prior to video assisted thoracoscopic surgery for the detection of small or deeply located lung nodules. We compared the localization ability of a mixture of lipiodol...
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Viability of preserved Cryptosporidium baileyi oocysts

Surl CG, Kim SM, Kim HC

The present study was undertaken to determine the viability and infectivity of oocysts of Cryptosporidium baileyi that had been stored from 1 to 40 months at 4 degrees C preserved...
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Prevalence of diarrhea caused by Cryptosporidium parvum in non-HIV patients in Jeollanam-do, Korea

Lee JK, Song HJ, Yu JR

The present study investigated the prevalence rate of Cryptosporidium parvum as a cause of diarrhea. We examined 942 stools of unidentified reasons occurring in patients in whom no immunosuppression had...
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MTT and Neutral red Biologic Assay for Human Bladder Cancer Cell Line(TCC-SuP) and Androgen Stimulated Cell Line of Mouse (SC-115): In Vitro Chemosensitivity Test

Kim BW, Kim DY, Chung SK, Chang SK

  • KMID: 2289661
  • Korean J Urol.
  • 1990 Oct;31(5):635-640.
To overcome the limitations of clonogenic assay (low plating efficiencies, clumping artifacts, long assay duration), we performed chemosensitivity test using MTT and neutral red biologic assay on SC-115 (androgen stimulated...
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Labeling Efficacy of Superparamagnetic Iron Oxide Nanoparticles to Human Neural Stem Cells: Comparison of Ferumoxides, Monocrystalline Iron Oxide, Cross-linked Iron Oxide (CLIO)-NH2 and tat-CLIO

Song M, Moon WK, Kim Y, Lim D, Song IC, Yoon BW

OBJECTIVE: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH2...
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Heterogeneity of the estrogen receptor in breast cancer tissues

Um JW, Bae JW, Lee JB, Kim HK, Koo BW

BACKGROUND: Popular immunohistochemical techniques for assay of estrogen receptor(ER) allow the localization of positive cells in specific cell populations. Some of breast carcinomas composed of discrete populations of cells were...
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