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Validation of SwimCountâ„¢, a Novel Home-Based Device That Detects Progressively Motile Spermatozoa: Correlation with World Health Organization 5th Semen Analysis

Yoon YE, Kim TY, Shin TE, Lee E, Choi KH, Lee SR, Hong YK, Park DS, Kim DK

PURPOSE: We evaluated the usefulness of a home-based device (SwimCountâ„¢) compared with World Health Organization (WHO) 5th semen analysis in screening for male fertility in Asian men. MATERIALS AND METHODS: One...
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Proteomic Signatures in Spermatozoa Reveal the Role of Paternal Factors in Recurrent Pregnancy Loss

Mohanty G, Jena SR, Nayak J, Kar S, Samanta L

PURPOSE: To identify the paternal factors responsible for aberrant embryo development leading to loss of foetus in recurrent pregnancy loss (RPL) through proteomic analysis of ejaculated spermatozoa. MATERIALS AND METHODS: This...
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Ritalinic Acid Stimulates Human Sperm Motility and Maintains Vitality In Vitro

Harlev A, Henkel R, Samanta L, Agarwal A

PURPOSE: To evaluate the in vitro impact of ritalinic acid (RA), a major metabolite of methylphenidate (drug to treat attention-deficit hyperactivity disorder), on sperm motility, vitality and oxidative stress. MATERIALS AND...
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Understanding the molecular mechanisms of bisphenol A action in spermatozoa

Rahman MS, Pang MG

Bisphenol A (BPA) is an endocrine-disrupting chemical that is capable of interfering with the normal function of the endocrine system in the body. Exposure to this chemical from BPA-containing materials...
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Validation of a smartphone-based, computer-assisted sperm analysis system compared with laboratory-based manual microscopic semen analysis and computer-assisted semen analysis

Cheon WH, Park HJ, Park MJ, Lim MY, Park JH, Kang BJ, Park NC

PURPOSE: This study aimed to evaluate the clinical efficacy of a portable smartphone-based system for computer-assisted semen analysis (CASA) compared with the results of manual microscopic semen analysis (SA) and...
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Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males

Dahril , Aulanni'am , Keumala V, Mustafa A

PURPOSE: Proprotein convertase subtilisin/kexin type 4 (PCSK4, a 54-kDa protease) is expressed in the plasma membrane of the acrosome in human spermatozoa. It plays a critical role in penetrating the...
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Does conventional freezing affect sperm DNA fragmentation?

Le MT, Nguyen TT, Nguyen TT, Nguyen TV, Nguyen TA, Nguyen QH, Cao TN

OBJECTIVE: Sperm cryopreservation has been widely used in assisted reproductive technology, as it offers great potential for the treatment of some types of male infertility. However, cryopreservation may result in...
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Evaluation of black glutinous rice (Oryza sativa L) extract as a novel nuclear stain for human sperm head assessment by microscopic examination

Chomean S, Sukanto T, Piemsup A, Chaiya J, Saenguthai K, Kaset C

OBJECTIVE: To compare black rice (Oryza sativa L) extract with three different staining methods for human sperm head assessment. METHODS: Semen samples were collected from 34 volunteers. Four smears of each...
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Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in In Vitro Fertilization and Intracytoplasmic Sperm Injection

Kim SM, Kim SK, Jee BC, Kim SH

PURPOSE: To investigate the associations between sperm DNA fragmentation (SDF) and embryo formation rate in normal responder women to in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). MATERIALS AND METHODS: Fifty-three consecutive, fresh...
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Long-Term Experience of Sperm Cryopreservation in Cancer Patients in a Single Fertility Center

Song SH, Kim DK, Sung SY, Her YS, Lee OH, Choi MH, Kim HK, Lyu SW, Kim DS

PURPOSE: Sperm cryopreservation before cancer treatment is the most effective method to preserve the fertility of male patients. We present our 21 years experience with sperm cryopreservation for cancer patients,...
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A point of confusion for embryologists in the identification of viable spermatozoa by the eosin-nigrosin test

Chen H, Zhou H, Shu J, Gan X, Wang C, Lin R

A viable spermatozoon is a prerequisite for fertilization in intracytoplasmic sperm injection (ICSI). Thus, it is crucial to select viable but immotile spermatozoa on the day of ICSI. We report...
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Evaluation of sperm DNA fragmentation using multiple methods: a comparison of their predictive power for male infertility

Javed A, Talkad MS, Ramaiah MK

OBJECTIVE: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to...
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Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation

Lee D, Jee BC

OBJECTIVE: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal...
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Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa

Kim SW, Nho EJ, Lee JY, Jee BC

OBJECTIVE: The aim of this study was to investigate DNA fragmentation status in human spermatozoa according to specific tail swelling patterns determined via hypo-osmotic swelling test (HOST). METHODS: Frozen semen samples...
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Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect

Bader R, Ibrahim JN, Mourad A, Moussa M, Azoury J, Azoury J, Alaaeddine N

BACKGROUND AND OBJECTIVES: Oxidative stress (OS) is known to be an important factor of male infertility. Adipose-derived mesenchymal stem cells (AD-MSCs) are known to have immune-modulatory and anti-oxidant effects through...
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ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively

Javed A, Ramaiah MK, Talkad MS

OBJECTIVE: The male reproductive system generates, accumulates, and transports the sperm. In this study, 2 methods of surgically retrieving sperm, namely, testicular sperm aspiration (TESA) and percutaneous epididymal sperm aspiration...
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Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm

Abdillah D, Setyawan E, Oh HJ, Ra K, Lee SH, Kim MJ, Lee BC

The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and...
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Testicular Fine-Needle Aspiration for Sperm Retrieval in Azoospermia: A Small Step toward the Technical Standardization

Cito G, Coccia ME, Sessa F, Cocci A, Verrienti , Picone R, Fucci R, Criscuoli , Serni S, Carini M, Natali A

PURPOSE: The aim was to describe our preliminary experience performing testicular fine-needle aspiration (TEFNA) with a larger needle in infertile patients with obstructive azoospermia, and to provide a systematic literature...
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What should be done for men with sperm DNA fragmentation?

Kim GY

In an age when a small quantity of sperm can lead to pregnancy through in vitro fertilization or intracytoplasmic sperm injection, selecting healthy sperm is important. Sperm DNA fragmentation (SDF)...
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Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters

Alkan I, Yüksel M, Canat HL, Atalay HA, Can O, Özveri H, Başar MM

PURPOSE: To investigate the pathophysiological role of superoxide anion and total reactive oxygen species (ROS) production by the spermatozoa of men with varicocele and its relationship with varicocele grade and...
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