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Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Seo JH, Yeo IS

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting anti-P. westermani specific Mab was...
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Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Yeo IS, Seo JH, Chang JK, Lee JS, Kim TS, Jeong GH

Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas...
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Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique

Joo KH, Hong SC, Chung MS, Rim HJ

Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the...
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Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis

Morakote N, Kong Y

Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,...
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Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis

Lee JH, Kong Y, Ryu JY, Cho SY

Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has...
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Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera

Lee SC, Chung YB, Kong Y, Kang SY, Cho SY

Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by...
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Detection of IgG and IgM antibodies with ELISA technique in human trichomoniasis

Yi MR, Shin MH, Leem MH, Ryu JS, Ahn MH, Min DY

The direct wet mount examination of vaginal secretion, widely applied for the diagnosis of Trichomonas vaginalis infection in woman patients, is rapid and economical, however, the sensitivity of this technique...
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Demonstration of species-specific and cross reactive components of Paragonimus westermani crude worm antigen by EITB

Joo KH, Ahn H, Chung MS, Rim HJ

Enzyme-linked immunoelectrotransfer blot (EITB) using crude worm antigen of adult Paragonimus westermani was performed for human patients sera to identify the species-specific components. Crude antigen was obtained by homogenizing and...
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The changes of histopathology and serum anti-sparganum IgG in experimental sparganosis of mice

Hong ST, Kim KJ, Huh S, Lee YS, Chai JY, Lee SH, Lee YS

The present study is intended to observe the chronologic changes of experimental sparganosis by histopathological observation and detection of circulating anti-sparganum IgG antibody using ELISA. Each of 25 mice was...
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