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Purification and characterization of recombinant murine endostatin in E. coli

You WK, So SH, Lee H, Park SY, Yoon MR, Chang SI, Kim HK, Joe YA, Hong YK, Chung SI

  • KMID: 1029927
  • Exp Mol Med.
  • 1999 Dec;31(4):197-202.
Endostatin, a carboxyl-terminal fragment of collagen XVIII is known as an anti-angiogenic agent, that specifically inhibits the proliferation of endothelial cell and the growth of several primary tumor....
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Reconstitution of class I MHC molecules expressed in E. coli and complexed with single antigenic peptides

Kim J, Kim MY, Chwae YJ, Park JH

The HLA-Cw3 heavy chain has been expressed at high level as insoluble protein aggregates in E. coli. The protein aggregates dissolved in strong denaturant solution were efficiently reconstituted by...
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Expression of a recombinant branched chain alpha-oxo acid dehydrogenase complex E2 (BCOADC-E2) in insect cells and its immunoreactivity to autoimmune sera

Lee SM, Ko SH, Park JE, Cha SH

  • KMID: 754990
  • Exp Mol Med.
  • 1998 Jun;30(2):65-71.
Preparation of a pure autoantigen by way of recombinant DNA technology has an important value in an accurate diagnosis or prognosis of an autoimmune disease. BCOADC-E2 subunit, a mitochondrial protein, has been...
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Functional activities of the Tsh protein from avian pathogenic Escherichia coli (APEC) strains

Kobayashi , Gaziri LC, Vidotto MC

The temperature-sensitive hemagglutinin (Tsh) expressed by strains of avian pathogenic Escherichia (E.) coli (APEC) has both agglutinin and protease activities. Tsh is synthesized as a 140 kDa precursor protein, whose...
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