- Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea
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Wu JD, Chou JC
- KMID: 2470181
- Mycobiology.
- 2019 Dec;47(4):483-493.
- doi: 10.1080/12298093.2019.1687252
Antrodia cinnamomea is a unique medicinal fungus in Taiwan. It has been found rich in some pharmacologically active compounds for anti-cancer, hangover, and immune regulation etc. With the in-depth study... -
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- Current Technologies and Related Issues for Mushroom Transformation
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Kim S, Ha BS, Ro HS
- KMID: 2312786
- Mycobiology.
- 2015 Mar;43(1):1-8.
- doi: 10.5941/MYCO.2015.43.1.1
Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign... -
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- Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes
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Kim JK, Park YJ, Kong WS, Kang HW
- KMID: 2312600
- Mycobiology.
- 2010 Dec;38(4):331-335.
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using... -
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- Isolation, Regeneration and PEG-Induced Fusion of Protoplasts of Pleurotus pulmonarius and Pleurotus florida
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Eyini M, Rajkumar K, Balaji P
- KMID: 2312482
- Mycobiology.
- 2006 Jun;34(2):73-78.
Inter-specific hybridization between Pleurotus pulmonarius and P. florida was attempted through PEG-induced protoplast fusion to select a fusant. The protocol for protoplast release, regeneration and fusion in these two Pleurotus... -
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- Isolation and Characterization of Dikaryotic Mutants from Pleurotus ostreatus by UV Irradiation
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Joh JH, Kim BG, Kong WS, Yoo YB, Chu KS, Kim NK, Park HR, Cho BG, Lee CS
- KMID: 1516940
- Mycobiology.
- 2004 Jun;32(2):88-94.
- doi: 10.4489/MYCO.2004.32.2.088
Protoplasts of the wild type strain of Pleurotus osteatus were mutagenized with UV light, and 3,000 colonies were examined for abnormal mycelial and fruiting phenotypes. Forty one strains displayed variant... -
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- The Efficient Transformation of Pleurotus ostreatus using REMI Method
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Joh JH, Kim BG, Chu KS, Kong WS, Yoo YB, Lee CS
- KMID: 2052977
- Mycobiology.
- 2003 Mar;31(1):32-35.
- doi: 10.4489/MYCO.2003.31.1.032
Restriction enzyme-mediated integration (REMI) was used to transform uracil auxotrophs of Pleurotus ostreatus to prototrophy. When protoplasts of Pleurotus ostreatus were treated by the reaction mixture containing 10 units of... -
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- Improved Transformation of the Filamentous Fungus Aspergillus niger Using Agrobacterium tumefaciens
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Park SM
- KMID: 2312346
- Mycobiology.
- 2001 Sep;29(3):132-134.
Since it is known that Agrobacterium tumefaciens, which has long been used to transform plants, can transfer the T-DNA to yeast Saccharomyces cerevisiae during tumourigenesis, a variety of fungi were... -
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- Ultrastructural Process of Protoplast Fusion Between Lentinula edodes and Coriolus versicolor
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Kim CK, Kim BK
- KMID: 2312337
- Mycobiology.
- 2001 Mar;29(1):15-18.
Protoplast fusion is a useful technique for establishing fungal hybrids to overcome the natural barriers. The ultrastructure of protoplast and its fusion process were observed using a scanning electron microscopy(SEM)... -
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- Improvement of Transformation System in Filamentous Fungus Aspergillus oryzae
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Lee JW, Yun CD, Hahm YT
- KMID: 2243203
- Korean J Med Mycol.
- 2001 Mar;6(1):1-8.
Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. A. oryzae is an important microorganism for industrial production of enzymes and fermented food products. It secrets large... -
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- Clinical utility of live blood analysis
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Kang JH, Sim JY, Cho HS, Ko DH, Kim SH, Lee HR
- KMID: 2295165
- J Korean Acad Fam Med.
- 2001 Jan;22(1):70-77.
BACKGROUND: Recently Live blood analysis was populated in korean society. so we evaluated clinical utility of Live blood analysis, as compared the Live blood analysis result of patients who have... -
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- Characteristics of Double Fungicide Resistant Strains of Venturia inaequalis
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Vijaya Palani P, Lalithakumari D
- KMID: 2052933
- Mycobiology.
- 2000 Jun;28(2):70-75.
Penconazole-resistant and cabendazim-resistant mutants of Venturiu inaequalis were developed by chemical (MNNG) mutagenesis. Protoplasts of these mutants were isolated and fused using polyethylene glycol as the fusogen. Fusants were classified... -
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- Cytological Study of the Introduction of Agrobacterium tumefaciens Spheroplasts into Nicotiana tabacum Protoplasts
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Kim JH, Koo YB, Lee KY
- KMID: 2373024
- Yeungnam Univ J Med.
- 1985 Dec;2(1):175-181.
- doi: 10.12701/yujm.1985.2.1.175
Agrobacterium tumefaciens induces cancerous growths called crown galls at wound sites on dicotyledonous plants. A large plasmid called T1 plasmid is responsible for virulence. Upon tumor induction, part of the... -
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