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A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms

Wang Y, Xu D, Liu D, Sun X, Chen Y, Zheng L, Chen L, Ma A

In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase...
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Development of a Molecular Marker Linked to the A4 Locus and the Structure of HD Genes in Pleurotus eryngii

Lee SH, Ali A, Ha B, Kim MK, Kong WS, Ryu JS

Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier...
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Development of a Novel Spawn (Block Spawn) of an Edible Mushroom, Pleurotus ostreatus, in Liquid Culture and its Cultivation Evaluation

Zhang WR, Liu SR, Kuang YB, Zheng SZ

Mushroom cultivation has gained increased attention in recent years. Currently, only four types of spawn, including sawdust spawn, grain spawn, liquid spawn, and stick spawn, are commonly available for mushroom...
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Yield, Nutritional Content, and Antioxidant Activity of Pleurotus ostreatus on Corncobs Supplemented with Herb Residues

Jin Z, Li Y, Ren J, Qin N

Improper disposal of herb residues in China has caused severe problems to the surrounding environment and human safety. Three herb residues, i.e., compound Kushen injection residues (CKI) and part one...
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Evaluation of Antioxidant, Anti-cholinesterase, and Anti-inflammatory Effects of Culinary Mushroom Pleurotus pulmonarius

Nguyen TK, Im KH, Choi J, Shin PG, Lee TS

Culinary mushroom Pleurotus pulmonarius has been popular in Asian countries. In this study, the anti-oxidant, cholinesterase, and inflammation inhibitory activities of methanol extract (ME) of fruiting bodies of P. pulmonarius...
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Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus

Song HY, Choi HJ, Jeong H, Choi D, Kim DH, Kim JM

A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent...
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The Effects of Different Substrates on the Growth, Yield, and Nutritional Composition of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus)

Hoa HT, Wang CL, Wang CH

The study was conducted to compare the effects of different agro-wastes on the growth, yield, and nutritional composition of oyster mushrooms Pleurotus ostreatus (PO) and Pleurotus cystidiosus (PC). Seven substrate...
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Differential Expression of Laccase Genes in Pleurotus ostreatus and Biochemical Characterization of Laccase Isozymes Produced in Pichia pastoris

Park M, Kim M, Kim S, Ha B, Ro HS

In this study, transcriptome analysis of twelve laccase genes in Pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. Lacc5 and Lacc12 were specifically expressed in...
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A Comprehensive Review of Tropical Milky White Mushroom (Calocybe indica P&C)

Subbiah KA, Balan V

A compressive description of tropical milky white mushroom (Calocybe indica P&C var. APK2) is provided in this review. This mushroom variety was first identified in the eastern Indian state of...
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The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus)

Hoa HT, Wang CL

The influences of temperature and nutritional conditions on the mycelium growth of oyster mushroom Pleurotus ostreatus (PO) and Pleurotus cystidiosus (PC) were investigated in laboratory experiment during the summer season...
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Anaphylaxis following mushrooms ingestion

Seo DH, Lee YS, Ban GY, Yoon MG, Kim JH, Shin YS, Park HS, Ye YM

Various foods can induce anaphylaxis. However, mushrooms-induced anaphylaxis has not been reported in Korea. We report a patient with past anaphylactic episode caused by mushroom ingestion, confirmed by the skin...
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Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus

Jang IT, Hyun SH, Shin JW, Lee YH, Ji JH, Lee JS

We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus...
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Overproduction of Laccase by the White-Rot Fungus Pleurotus ostreatus Using Apple Pomace as Inducer

Park YJ, Yoon DE, Kim HI, Kwon OC, Yoo YB, Kong WS, Lee CS

  • KMID: 2312745
  • Mycobiology.
  • 2014 Jun;42(2):193-197.
Laccase activity of Pleurotus ostreatus is significantly increased by the addition of apple pomace. Among various conditions, the best concentration of apple pomace and cultivation time for the production of...
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Isolation of a Variant Strain of Pleurotus eryngii and the Development of Specific DNA Markers to Identify the Variant Strain

Lee HJ, Kim SW, Ryu JS, Lee CY, Ro HS

  • KMID: 2312722
  • Mycobiology.
  • 2014 Mar;42(1):46-51.
A degenerated strain of Pleurotus eryngii KNR2312 was isolated from a commercial farm. Random amplified polymorphic DNA analysis performed on the genomic DNA of the normal and degenerated strains of...
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Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers

Kim SW, Kim S, Lee HJ, Park JW, Ro HS

  • KMID: 2312712
  • Mycobiology.
  • 2013 Dec;41(4):252-255.
Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four...
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Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

Lim SH, Lee YH, Kang HW

  • KMID: 2312705
  • Mycobiology.
  • 2013 Dec;41(4):214-220.
This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost...
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Anticancer and Immunopotentiating Activities of Crude Polysaccharides from Pleurotus nebrodensis on Mouse Sarcoma 180

Cha YJ, Alam N, Lee JS, Lee KR, Shim MJ, Lee MW, Kim HY, Shin PG, Cheong JC, Yoo YB, Lee TS

Pleurotus nebrodensis is an edible and commercially available mushroom in Korea. This study was conducted in order to evaluate the anticancer and immunopotentiating activities of crude polysaccharides, extracted in methanol,...
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Characterization of Species of Cladobotryum which Cause Cobweb Disease in Edible Mushrooms Grown in Korea

Back CG, Lee CY, Seo GS, Jung HY

  • KMID: 2312672
  • Mycobiology.
  • 2012 Sep;40(3):189-194.
Four Cladobotryum isolates were collected from four different commercially grown mushroom types infected with cobweb disease in Cheongdo-gun and Chilgok-gun of Gyeongbuk Province, Korea in 2010. The isolates were identified...
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Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes

Chukeatirote E, Maharachchikumbura SSN, Wongkham S, Sysouphanthong P, Phookamsak R, Hyde KD

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA...
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Anti-leukemic and immunomodulatory effects of fungal metabolites of Pleurotus pulmonarius and Pleurotus ostreatus on benzene-induced leukemia in Wister rats

Olufemi AE, Terry AO, Kola OJ

BACKGROUND: The use of natural bioactive compounds in conventional chemotherapy is a new direction in cancer treatment that is gaining more research attention recently. Bioactive polysaccharides and polysaccharide-protein complexes from...
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