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High mRNA expression of GABA receptors in human sperm with oligoasthenoteratozoospermia and teratozoospermia and its association with sperm parameters and intracytoplasmic sperm injection outcomes

Kaewman P, Nudmamud-Thanoi S, Amatyakul P, Thanoi S

Objective: This study investigated the mRNA expression of gamma-aminobutyric acid (GABA) receptors in the sperm of oligoasthenoteratozoospermic (OAT) and teratozoospermic (TER) men compared to normozoospermic (NOR) men, as well as...
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Semen parameters on the intracytoplasmic sperm injection day: Predictive values and cutoff thresholds of success

Moubasher AEdAA, Taha EA, Elnashar EM, Abdel Maged AAA, Zahran AM, Sayed HH, Gaber HD

Objective: This study was conducted to investigate the relationship of semen parameters in samples used for intracytoplasmic sperm injection (ICSI) with fertilization and pregnancy rates in infertile couples. Methods: In this...
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Effects of prematuration culture with a phosphodiesterase-3 inhibitor on oocyte morphology and embryo quality in in vitro maturation

Cheruveetil MA, Shetty PK, Rao KA, Rajendran A, Asif M

Objective: The study assessed the developmental potential of germinal vesicle (GV) oocytes subjected to in vitro maturation (IVM) after prematuration culture with cilostamide (a phosphodiesterase-3 inhibitor) and the impact of...
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Effect of in vitro testicular spermatozoa culture on pregnancy outcomes: an experience at a single university hospital

Lee J, Yoo JH, Lee JH, Ahn HS, Hwang KJ, Kim M

Background: There are no guidelines for the optimal incubation time or temperature to improve pregnancy outcomes in testicular sperm extraction-intracytoplasmic sperm injection (TESE-ICSI) cycles. We aimed to evaluate whether a...
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Hepatitis B Surface Antigen Seropositive Men in Serodiscordant Couples: Effects on the Assisted Reproductive Outcomes

Cito G, Coccia ME, Fucci R, Picone R, Cocci A, Sessa M, Sessa F, Rizzello F, Micelli E, Trotta M, Badolato L, Campi R, Criscuoli L, Serni S, Carini M, Natali A

Purpose: To evaluate the influence of hepatitis B virus (HBV) infection in men of serodiscordant couples on the reproductive outcomes. Materials and Methods: A total of 134 infertile couples were included...
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The effect of insemination methods on in vitro maturation outcomes

Pongsuthirak P

Objective: The aim of this study was to compare the effects of conventional insemination (in vitro fertilization [IVF]) and intracytoplasmic sperm injection (ICSI) on the fertilization, developmental competence, implantation potential,...
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A point of confusion for embryologists in the identification of viable spermatozoa by the eosin-nigrosin test

Chen H, Zhou H, Shu J, Gan X, Wang C, Lin R

A viable spermatozoon is a prerequisite for fertilization in intracytoplasmic sperm injection (ICSI). Thus, it is crucial to select viable but immotile spermatozoa on the day of ICSI. We report...
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Comparison of clinical outcomes between in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in IVF-ICSI split insemination cycles

Lee SH, Lee JH, Park YS, Yang KM, Lim CK

OBJECTIVE: This study aimed to compare the clinical outcomes between in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in sibling oocytes. Additionally, we evaluated whether the implementation of split...
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Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

Kim HJ, Yoon HJ, Jang JM, Lee WD, Yoon SH, Lim JH

OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830...
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Influence of the insemination method on the outcomes of elective blastocyst culture

Wang C, Feng G, Zhang B, Shu J, Zhou H, Gan X, Lin R

OBJECTIVE: The aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture. METHODS: We retrospectively analyzed the outcomes of elective blastocyst...
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The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles

Fesahat F, Dehghani Firouzabadi R, Faramarzi , Khalili MA

OBJECTIVE: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate...
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A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser

Chen H, Feng G, Zhang B, Zhou H, Shu J, Gan X

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of...
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Follicular fluid cerebellin and betatrophin regulate the metabolic functions of growing follicles in polycystic ovary syndrome

Ersahin AA, Acet M, Ersahin SS, Acet T, Yardim M, Kenanoglu , Aydin S

OBJECTIVE: The aim of this study was to assess the changes of follicular fluid (FF) and serum levels of cerebellin precursor protein 1 (cbln1) and betatrophin in patients with polycystic...
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Relationship between meiotic spindles visualization and intracytoplasmic sperm injection outcomes in human oocytes

Asa E, Tabatabaee R, Farrokhi A, Nejatbakhsh R

In assisted reproductive techniques, the operator attempts to select morphologically best embryos to predict embryo viability. Development of polarized light microscope, which evaluates the oocytes' spindles according to birefringence of...
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Congenital Malformations in Infants of Mothers Undergoing Assisted Reproductive Technologies: A Systematic Review and Meta-analysis Study

Hoorsan H, Mirmiran P, Chaichian , Moradi Y, Hoorsan R, Jesmi F

OBJECTIVES: This meta-analysis aimed to evaluate congenital malformations in infants conceived by assisted reproductive techniques (ART), compared with infants conceived spontaneously. METHODS: In this study, available resources searched to find relevant...
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Does intrauterine injection of low-molecular-weight heparin improve the clinical pregnancy rate in intracytoplasmic sperm injection?

Kamel AM, El-Faissal Y, Aboulghar , Mansour R, Serour GI, Aboulghar

OBJECTIVE: Heparin can modulate proteins, and influence processes involved in implantation and trophoblastic development. This study aimed to assess the improvement of clinical pregnancy and implantation rates after local intrauterine...
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Effects of maternal age on embryo quality and pregnancy outcomes using testicular sperm with intracytoplasmic sperm injection

Choi HW, Park YS, Lee SH, Lim CK, Seo JT, Yang KM

OBJECTIVE: The aim of this study was to evaluate the influence of maternal age on fertilization, embryo quality, and clinical pregnancy in patients undergoing intracytoplasmic sperm injection (ICSI) using testicular...
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Pregnancy and fertilization potential of immature oocytes retrieved in intracytoplasmic sperm injection cycles

Ko DS, Lee SH, Park DW, Yang KM, Lim CK

OBJECTIVE: The goal of this study was to evaluate the pregnancy potential of immature (metaphase I or germinal vesicle stage) oocytes retrieved in intracytoplasmic sperm injection (ICSI) cycles. METHODS: A total...
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The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study

Jahromi BN, Mosallanezhad Z, Matloob N, Davari M, Ghobadifar MA

OBJECTIVE: In order to increase the number of mature oocytes usable for intracytoplasmic sperm injection (ICSI), we aimed to investigate the effect of co-culturing granulosa cells (GCs) on human oocyte...
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Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in human in vitro fertilization

Kang HJ, Lee SH, Park YS, Lim CK, Ko DS, Yang KM, Park DW

OBJECTIVE: Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce...
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