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Application of bispecific antibody against antigen and hapten for immunodetection and immunopurification

Kim H, Park S, Lee HK, Chung J

  • KMID: 2154262
  • Exp Mol Med.
  • 2013 Sep;45(9):e43.
We present a bispecific antibody that recognizes an antigen and a hapten and can be applied to various biological assays, including immunoblotting and immunoprecipitation. In immunoblot analysis of serum, an...
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Diagnostic value of a dot immunobinding assay for human pulmonary hydatidosis

Olut AI, Erguven S, Emri S, Ozunlu H, Akay H

The diagnosis of human hydatidosis is primarily made using radiological and serological methods. Radiological methods are generally of low specificity and serological methods lack sensitivity, especially for pulmonary disease. In...
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Dot-Blot Immunoassay of Fasciola gigantica Infection using 27 kDa and Adult Worm Regurge Antigens in Egyptian Patients

Kamel HH, Saad GA, Sarhan RM

The purpose of the present study was to evaluate the potential role of the 27-Kilodalton (KDa) antigen versus Fasciola gigantica adult worm regurge antigens in a DOT-Blot assay and to...
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Evaluation of Anti-dsDNA Antibody Tests: Crithidia luciliae Immunofluorescence Test, Immunoblot, Enzyme-linked Immunosorbent Assay, Chemiluminescence Immunoassay

Yang JY, Oh EJ, Kim Y, Park YJ

BACKGROUND: Anti-double stranded DNA antibody (anti-dsDNA) test is useful for the diagnosis and monitoring of systemic lupus erythematosus (SLE). Although several methods are available, none of them is completely satisfactory...
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Allergen Specific IgE Measurement with Polycheck Allergy: Comparison of Three Multiple Allergen Simultaneous Tests

Jang WR, Nahm CH, Kim JH, Lim DH, Jang TY, Moon YS, Kim JJ

BACKGROUND: The in vivo skin prick test (SPT) or in vitro detection of allergen specific IgE in serum is commonly used for the diagnosis of allergic disease. In this study,...
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