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Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography

Kong Y, Kang SY, Cho SY

Out of many component proteins in crude saline extract of Spirometra mansoni plerocercoid (sparganum), 36 kDa and 29 kDa proteins were found to be the most antigenic and were already...
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Tissue origin of soluble component proteins in saline extract of adult Paragonimus westermani

Kong Y, Park CY, Kang SY, Cho SY

Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf...
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Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis

Morakote N, Kong Y

Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,...
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Protein composition and antigenicity of the tegument from Paragonimus westermani

Kim SI, Cho SY

To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component...
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Immunohistochemical localization of 36 and 29 kDa proteins in sparganum

Kim LS, Kong Y, Kang SY, Cho SY

Antigenic proteins of 36 and 29 kDa were localized in Spirometra mansoni plerocercoid (sparganum) immunohistochemically by avidin biotin complex (ABC) staining. When polyclonal antibodies such as BALB/c mouse serum immunized...
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Component proteins and protease activities in excretory-secretory product of sparganum

Cho SY, Chung YB, Kong Y

Spirometra mansoni plerocercoid (sparganum) was incubated in saline at 4 degrees C or 37 degrees C up to 100 hours. Protein contents in the excretory-secretory product (ESP) were rather constant...
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Molecular weight of major component proteins in crude saline extract of adult Paragonimus westermani

Kong Y, Kim WB, Kang SY, Cho SY

When the component proteins in crude saline extract of 13-week old adult Paragonimus westermani were observed by non-denaturing discontinuous-polyacrylamide gel electrophoresis (Disc-PAGE), 8 distinct bands were clearly recognized. Molecular weight...
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Component proteins in crude extract of adult Paragonimus westermani purified by immunoaffinity chromatography using monoclonal antibodies

Kang SY, Kong Y, Cho SY

The nature of 2 component proteins in crude saline extract of adult Paragonimus westermani was investigated. By immunoaffinity chromatography using monoclonal antibodies (MAb) as ligands, the proteins were purified from...
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Immunoelectrophoretic analysis of major component proteins in cystic fluid of Taenia solium metacestodes

Kong Y, Cho SY, Kim SI, Kang SY

When cystic fluid of Taenia solium metacestodes (CF) was filtrated through Sephacryl S-300 Superfine, major proteins were in fractions III and IV. Major protein in fraction III was Band C...
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Measurement of 150 kDa protein of Taenia solium metacestodes by antibody-sandwich ELISA in cerebrospinal fluid of neurocysticercosis patients

Cho SY, Kong Y, Kim SI, Kang SY

An antigenic protein in cystic fluid of Taenia solium metacestodes (CF) of 150 kDa was measured by antibody-sandwich ELISA in serum and cerebrospinal fluid (CSF) of neurocysticercosis patients. Capture antibodies...
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Component proteins in cystic fluid of Taenia solium metacestodes collected surgically from neurocysticercosis patients

Kong Y, Kang SY, Cho SY

Surgically collected cystic fluid of Taenia solium metacestodes from patients of intracranial cystic lesion were compared in their protein composition with those from naturally infected pigs in Cheju Do, Korea...
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