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Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model

He XY, Ma LB, He XN, Si WT, Zheng YM

Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target...
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In Vitro Infectivity Assessment by Drug Susceptibility Comparison of Recombinant Leishmania major Expressing Enhanced Green Fluorescent Protein or EGFP-Luciferase Fused Genes with Wild-Type Parasite

Sadeghi S, Seyed N, Etemadzadeh MH, Abediankenari S, Rafati S, Taheri T

Leishmaniasis is a worldwide uncontrolled parasitic disease due to the lack of effective drug and vaccine. To speed up effective drug development, we need powerful methods to rapidly assess drug...
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Mouse Fyn induces pseudopodium formation in Chinese hamster ovary cells

An L, Liu S, Zhang W, Zhang Y, Huang Y, Hu X, Chen S, Zhao S

Molecular mechanisms underlying the effects of Fyn on cell morphology, pseudopodium movement, and cell migration were investigated. The Fyn gene was subcloned into pEGFP-N1 to produce pEGFP-N1-Fyn. Chinese hamster ovary...
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Establishment of an Orthotopic Mouse Non-Muscle Invasive Bladder Cancer Model Expressing the Mammalian Target of Rapamycin Signaling Pathway

Kim SJ, Seo HK, Seo HH, Lee SJ, Kwon JK, Lee TJ, Chi BH, Chang IH

We established an orthotopic non-muscle invasive bladder cancer (NMIBC) mouse model expressing the mammalian target of the rapamycin (mTOR) signaling pathway. After intravesical instillation of KU-7-lucs (day 0), animals were...
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Gene transfer using liposome-complexed adenovirus seems to overcome limitations due to coxsackievirus and adenovirus receptor-deficiency of cancer cells, both in vitro and in vivo

Han SY, Lee YJ, Jung HI, Lee SW, Lim SJ, Hong SH, Jeong JS

Use of adenoviruses as vehicle for gene therapy requires that target cells express appropriate receptors such as coxsakievirus and adenovirus receptor (CAR). We show here that CAR-deficiency in cancer cells,...
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Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells

Ahn HJ, Kim S, Nam HW

Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar...
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Expression of Hepatitis C Virus Core Protein in Hepatocytes Does Not Modulate Proliferation or Apoptosis of CD8+ T Cells

Jin YH, Crispe IN, Park S

  • KMID: 2428300
  • Yonsei Med J.
  • 2005 Dec;46(6):827-834.
Hepatocytes are the primary targets of the hepatitis C virus (HCV). While immunosuppressive roles of HCV core protein have been found in several studies, it remains uncertain whether core protein...
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Efficient gene delivery in differentiated human embryonic stem cells

Kim JH, Do HJ, Choi SJ, Cho HJ, Park KH, Yang HM, Lee SH, Kim DK, Kwack K, Oh SK, Moon SY, Cha KY, Chung HM

  • KMID: 1033099
  • Exp Mol Med.
  • 2005 Feb;37(1):36-44.
Human embryonic stem (hES) cells are capable of differentiating into pluralistic cell types, however, spontaneous differentiation generally gives rise to a limited number of specific differentiated cell types and a...
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