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Cell-mediated immunity in experimental amoebic meningoencephalitis

Im KI, Chung PR, Kim TU

Cell-mediated and humoral immune reactions in mice infected with pathogenic Acanthamoeba culbertsoni were observed according to the period of time after amoebic infection by intranasal inoculation. The degrees of blastogenesis...
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Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay

Choi WY, Nam HW, Youn JH, Kim DJ, Kong Y, Kang SY, Cho SY

Sensitivity of anti-Toxoplasma antibody (IgG) test by enzyme-linked immunosorbent assay (ELISA) was evaluated in comparison with indirect latex agglutination (ILA) using 2,016 paired human samples of serum and cerebrospinal fluid...
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Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody

Cho SY, Kang SY, Kong Y

The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine....
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Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis

Lee JH, Kong Y, Ryu JY, Cho SY

Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has...
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IgA response in mice infected with Neodiplostomum seoulensis

Huh S, Lee SU, Won MH, Jeong YG, Kwon YH, Choi CS

To observe the production of IgA in Balb/c mice with neodiplostomiasis, 20 mice were infected with each 200 metacercariae of Neodiplostomum seoulenis. Sera and the duodenums were obtained 3, 7,...
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Detection of IgG and IgM antibodies with ELISA technique in human trichomoniasis

Yi MR, Shin MH, Leem MH, Ryu JS, Ahn MH, Min DY

The direct wet mount examination of vaginal secretion, widely applied for the diagnosis of Trichomonas vaginalis infection in woman patients, is rapid and economical, however, the sensitivity of this technique...
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ELISA of rat sera infected with Paragonimus iloktsuenensis

Lim BK, Lee OR, Nam HS

Enzyme-linked immunosorbent assay (ELISA) of paragonimiasis iloktsuenensis rat sera was performed using crude antigens of Paragonimus iloktsuenensis (PIA), P. westermani (PWA) and Clonorchis sinensis (CSA). Three crude antigens (PIA, PWA,...
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Antibody changes in paragonimiasis patients after praziquantel treatment as observed by ELISA and immunoblot

Cho SY, Kim SI, Kang SY, Kong Y, Han SK, Shim YS, Han YC

To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels...
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Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis

Morakote N, Kong Y

Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,...
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Measurement of 150 kDa protein of Taenia solium metacestodes by antibody-sandwich ELISA in cerebrospinal fluid of neurocysticercosis patients

Cho SY, Kong Y, Kim SI, Kang SY

An antigenic protein in cystic fluid of Taenia solium metacestodes (CF) of 150 kDa was measured by antibody-sandwich ELISA in serum and cerebrospinal fluid (CSF) of neurocysticercosis patients. Capture antibodies...
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Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography

Kong Y, Kang SY, Cho SY

Out of many component proteins in crude saline extract of Spirometra mansoni plerocercoid (sparganum), 36 kDa and 29 kDa proteins were found to be the most antigenic and were already...
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Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera

Lee SC, Chung YB, Kong Y, Kang SY, Cho SY

Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by...
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Serologic response to Pneumocystis carinii of Seoul National University Hospital patients

Hong ST

A total of 2,580 sera of the patients who were consulted to the serology laboratory of the Seoul National University Hospital were collected in 1990. The sera were screened by...
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Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Seo JH, Yeo IS

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting anti-P. westermani specific Mab was...
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Changes of IgM and IgG antibody levels in experimental rabbit anisakiasis as observed by ELISA and SDS-PAGE/immunoblot

Yang HJ, Cho YJ, Paik YH

Antibody changes in experimental anisakiasis were observed in 10 rabbits which were infected each with 10 Anisakis simplex larvae. The sera were collected before and on the 6th to the...
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Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Yeo IS, Seo JH, Chang JK, Lee JS, Kim TS, Jeong GH

Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas...
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Immunological approach for classification of free-living amoeba in Korea

Shin HJ, Kim CH, Im KI

Acanthamoeba spp., free-living amoebae inhabited in moist soil, pond, freshwater, sewage, atmosphere and swimming pool, may be causative protozoa of the fatal primary amoebic meningoencephalitis in experimental animals and humans....
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The fate of spargana inoculated into the cat brain and sequential changes of anti-sparganum IgG antibody levels in the cerebrospinal fluid

Wang KC, Huh S, Hong ST, Chai JY, Choi KS, Lee SH

To establish an animal model of intracranial sparganosis, the fate and behavior of the experimentally inoculated spargana were observed. A total of 102 scolices of spargana were injected into 22...
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Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies

Yong TS, Im K, Chung PR

Clonorchis sinensis is a common parasite of man in Korea. Researches on the specific antigens of C. sinensis would be valuable not only because those elucidate the molecular characteristics of...
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Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium

Kong Y, Kang SY, Cho SY, Min DY

Sera from confirmed patients of 5 hydatidosis, 67 neurocysticercosis and 89 other parasitic diseases were tested for specific antibody (IgG) levels by ELISA to cystic fluid antigens from metacestodes of...
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