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Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus

Lee WJ, Shin MK, Cha SB, Yoo HS

Swine hepatitis E virus (HEV) is widespread throughout pigs in both developing and industrialized countries. This virus is an important zoonotic agent and a public concern worldwide. Infected pigs are...
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Comparison Study with Enzyme Immunoassay and Chemiluminescence Immunoassay for Hepatitis B Virus Surface Antigen Detection

Huh HJ, Chae SL, Cha YJ

BACKGROUND: The serological detection of the surface antigen (HBsAg) of hepatitis B virus (HBV) is the basis of detection of HBV infections in blood donors and patients with hepatitis....
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Development of a Quantitative Sandwich Enzyme-Linked Immunosorbent Assay for Detecting the MPT64 Antigen of Mycobacterium tuberculosis

Ji M, Cho B, Cho YS, Park SY, Cho SN, Jeon BY, Yoon BS

PURPOSE: Tuberculosis (TB) is a major infectious disease and is responsible for two million deaths annually. For the identification and quantitation of Mycobacterium tuberculosis (M. tuberculosis), a causative agent of...
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Estimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian Approach

Pak SI, Kim D, Salman M

  • KMID: 1099821
  • J Vet Sci.
  • 2003 Apr;4(1):51-56.
To draw inferences about the sensitivity and specificity of the newly developed ELISA test for bovine paratuberculosis (PTB) diagnosis and posterior distribution on the prevalence of PTB in a province...
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Comparison of Two Enzyme Immunoassay for Detection of Clostridium difficile Toxin A and Toxin B

Shin BM, Yoo SJ, Oh HJ

BACKGROUND: Enzyme immunoassay (EIA) capable of detecting both toxin A and toxin B is strongly recommended for the diagnosis of Clostridium difficile associated disease. Therefore, we evaluated two different EIAs...
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Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay

Lee HM, Song SO, Cha SH, Wee SB, Bischoff K, Park SW, Son SW, Kang HG, Cho MH

Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative...
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Evaluation of Anti-dsDNA Antibody Tests: Crithidia luciliae Immunofluorescence Test, Immunoblot, Enzyme-linked Immunosorbent Assay, Chemiluminescence Immunoassay

Yang JY, Oh EJ, Kim Y, Park YJ

BACKGROUND: Anti-double stranded DNA antibody (anti-dsDNA) test is useful for the diagnosis and monitoring of systemic lupus erythematosus (SLE). Although several methods are available, none of them is completely satisfactory...
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Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay

Cha SH, Kim SH, Bischoff K, Kim HJ, Son SW, Kang HG

A monoclonal antibody (mAb) against zearalenone (ZEN) was produced using ZEN-carboxymethoxylamine and -BSA conjugates. Antibody produced by one clone showing a very high binding ability was selected and found to...
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Efficacy of the Merozoite Surface Protein 1 of Plasmodium Vivax as an Antigen for ELISA to Diagnose Malaria

Kim YM, Hwang HA, Yun WS, Kim SI, Lee KW, Park SK, Lee YJ, Kim TK, Wongsrichanalai C, Sakanari J, Park H

Malaria is still a major health problem in Thailand and its incidence is currently rising in Korea. To identify a useful antigen for the diagnosis of malaria patients, a cDNA...
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Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies

Kim SH, Park MK, Kim JY, Chuong PD, Lee YS, Yoon BS, Hwang KK, Lim YK

  • KMID: 1106073
  • J Vet Sci.
  • 2005 Mar;6(1):41-46.
Five monoclonal antibodies (MAbs) and chicken immunoglobulin (IgY) were developed by immunizing with flagella purified from Listeria monocytogenes 4b and the five MAbs have been confirmed to be specific against...
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Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

Choi IS, Yoo HS

  • KMID: 1095123
  • J Vet Sci.
  • 2002 Sep;3(3):207-212.
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2...
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Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

Choi MH, Park IC, Li S, Hong ST

Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from...
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Evaluation of Two ELISA and Two Indirect Hemagglutination Tests for Serodiagnosis of Pulmonary Hydatid Disease

Eris FN, Akisu C, Aksoy U

To establish a definite diagnosis for pulmonary hydatid disease, combination of radiology and serology is useful. In this study, 19 preoperative sera from patients with surgically confirmed pulmonary hydatidosis, 40...
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Comparisons of Latex Agglutination, Immunochromatography and Enzyme Immunoassay Methods for the Detection of Rotavirus Antigen

Lee SY, Hong JH, Lee SW, Lee M

BACKGROUND: Rotavirus is the most common cause of childhood gastroenteritis during winter season. Rapid, accurate diagnosis is essential for preventing severe complications of rotaviral gastroenteritis. The sensitivity and specificity...
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Comparative Evaluation of ELISA and Luminex Panel Reactive Antibody Assays for HLA Alloantibody Screening

Jung S, Oh EJ, Yang CW, Ahn WS, Kim Y, Park YJ, Han K

BACKGROUND: For the detection of HLA antibodies, solid-phase tests using purified HLA antigens are increasingly used. In this study, we analyzed the panel reactive antibody (PRA) test results using ELISA...
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Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae

Jin Y, Shen C, Huh S, Sohn WM, Choi MH, Hong ST

Toxocariasis is a worldwide zoonosis caused by larvae of ascarid nematodes of dogs or cats, Toxocara canis or T. cati. Diagnosis of human toxocariasis currently relies on serology that uses...
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Utility of ELISA Optical Density Values and Clinical Scores for the Diagnosis of and Thrombosis Prediction in Heparin-induced Thrombocytopenia

Kim SY, Kim HK, Han KS, Kim I, Yoon SS, Park S, Kim BK

BACKGROUND: Heparin-induced thrombocytopenia (HIT) is an adverse drug reaction caused by antibodies to the heparin/platelet factor 4 (PF4) complex, resulting in thrombocytopenia and prothrombotic state. HIT diagnosis is challenging and...
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A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran

Motevalli Haghi A, Khoramizade MR, Nateghpour M, Mohebali M, Edrissian , Eshraghian MR, Sepehrizadeh Z

In Iran, Plasmodium vivax is responsible for more than 80% of the infected cases of malaria per year. Control interventions for vivax malaria in humans rely mainly on developed diagnostic...
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