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Comparative Evaluation of the STANDARD F Influenza A/B FIA Test with the Sofia Influenza A+B FIA and SD BIOLINE Influenza Ag A/B/A(H1N1) tests for Influenza A Virus Detection

Yoo S, Kim SH, Jeon GW, Park YH, Song SA, Shin JH

Background: This study aimed to evaluate the diagnostic performance of the STANDARD F Influenza A/B FIA test (SD Biosensor Inc., Korea) for the rapid detection of influenza A virus in...
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Laboratory Diagnosis of Clostridium difficile Infection in Korea: The First National Survey

Chung HS, Park JS, Shin BM

In May 2015, we conducted a voluntary online survey on laboratory diagnostic assays for Clostridium difficile infection (CDI) across clinical microbiology laboratories in Korea. Responses were obtained from 66 laboratories,...
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Performance Evaluation of Two Immunochromatographic Assays for Rotavirus Detection in Stool Specimens

Kim S, Kim YJ, Cho SY, Park TS, Lee HJ

BACKGROUND: Rotavirus is a major pathogen causing enteritis worldwide in children under five years of age. In recent years, immunochromatographic assay (ICA) has been widely used as a diagnostic test...
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Application of a Grey Zone for HCV and HIV Immunoassays for Blood Donor Screening: Is It still Necessary?

Cho YJ, Kwon SY, Yang JH, Kim J, Kim BH, Shin S

BACKGROUND: When enzyme immunoassays (EIA) were implemented, considering the limited sensitivity of 1st generation EIAs, the Korean Red Cross (KRC) applied grey zones for detection of weak reactive samples that...
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Evaluation of a Rapid Membrane Enzyme Immunoassay for the Simultaneous Detection of Glutamate Dehydrogenase and Toxin for the Diagnosis of Clostridium difficile Infection

Kim H, Kim WH, Kim M, Jeong SH, Lee K

We evaluated the new C. DIFF QUIK CHEK COMPLETE (CD COMPLETE; TechLab, USA), which is a rapid membrane enzyme immunoassay that uses a combination of glutamate dehydrogenase (GDH) antigen and...
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Performance Evaluation of 3 Kinds of HBsAg Qualitative Assays and 2 Kinds of Quantitative Assays

Park JS, Choi JI, Lim JH, Jung J, Lee SH, Park NH, Shin JW, Nah YW, Nam CW, Cha YJ, Kim SR

BACKGROUND: Currently used techniques for quantitation of HBsAg often yield discordant results; therefore, development of quantitation techniques that can detect HBsAg with high accuracy has become very important. Recent advances...
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Performance Evaluation of Immunoassay Detection of HBsAg Mutants and Their Clinical Significance in the High Risk Groups

Choi JI, Lim JH, Jeong J, Lee SH, Park JS, Park NH, Shin JW, Nah YW, Nam CW, Cha YJ, Kim SR

  • KMID: 2312217
  • Lab Med Online.
  • 2013 Apr;3(2):88-96.
BACKGROUND: False negative results have been reported in the immunodetection of hepatitis B virus (HBV) because of the existence of the various mutants of the virus, causing most suppliers to...
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Evaluation of the Xpert Clostridium difficile Assay for the Diagnosis of Clostridium difficile Infection

Shin S, Kim M, Kim M, Lim H, Kim H, Lee K, Chong Y

Infection with Clostridium difficile is a growing concern because of the increasing prevalence and spread of nosocomial infections. Emergence of the hypervirulent 027/NAP1/BI strain is also notable. Existing diagnostic methods...
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Evaluation of the Chorus Mycoplasma pneumoniae IgM Assay for the Serological Diagnosis of Mycoplasma pneumoniae Infection

Kim S, Um TH, Cho CR

  • KMID: 1820224
  • J Lab Med Qual Assur.
  • 2012 Jun;34(1):57-62.
BACKGROUND: Mycoplasma pneumoniae is the most frequent cause of respiratory tract infections in school-aged children and adolescents. M. pneumoniae infection has variable clinical manifestations and is resistant to beta-lactam antibiotics,...
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The Comparison between Particle Agglutination Assay and Enzyme Immunoassay in the Detection of Mycoplasma pneumoniae-Specific Antibody

Jung HW, Jearn LH, Kim TY

BACKGROUND: The two common serological test methods used for initial diagnosis of acute Mycoplasma pneumoniae (MP) pneumonia are particle agglutination assay (PA) and enzyme immunoassay (EIA). We compared the differences...
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Seroepidemiologic Survey of Varicella-Zoster Virus in Korean Adults Using Glycoprotein Enzyme Immuno Assay and Fluorescent Antibody to Membrane Antigen Test

Kim YH, Hwang JY, Lee KM, Choi JH, Lee TY, Choi JS, Park HS

BACKGROUND: Herpes zoster (HZ) occurs mainly in the elderly and Korea is rapidly becoming an aging society. Therefore, it is important to know the immune status against varicella-zoster virus (VZV)...
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Comparison of Two Enzyme Immunoassay for Detection of Clostridium difficile Toxin A and Toxin B

Shin BM, Yoo SJ, Oh HJ

BACKGROUND: Enzyme immunoassay (EIA) capable of detecting both toxin A and toxin B is strongly recommended for the diagnosis of Clostridium difficile associated disease. Therefore, we evaluated two different EIAs...
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Significance of Anti-HCV Signal-to-Cutoff Ratio in Predicting Hepatitis C Viremia

Seo YS, Jung ES, Kim JH, Jung YK, Kim JH, An H, Yim HJ, Yeon JE, Byun KS, Kim CD, Ryu HS, Um SH

BACKGROUND/AIMS: Hepatitis C virus (HCV) RNA testing can be performed using qualitative or quantitative assays, and it is still unclear which is more useful as a primary test in patients...
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Usefulness of Short-term Follow-up Enzyme Immunoassay in Mycoplasma pneumoniae Infection

Seo BO, Kang JW, Kim BG, Lee EY, Kim SW

  • KMID: 2314907
  • Pediatr Allergy Respir Dis.
  • 2008 Dec;18(4):305-315.
PURPOSE: The diagnosis of Mycoplasma pneumoniae (M. pneumoniae) infection is usually based on serology using complement fixation assay (CFA), particle agglutination test (PA), enzyme immunoassay (EIA) and polymerase chain reaction...
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Evaluation of Rapid Assay (Tox A/B Quik Chek) for the Detection of Clostridium difficile Toxins A and B

Kim SJ, Kim H, Kim MS, Koh E, Kim CK, Jeong SH, Chong Y, Lee K

BACKGROUND: Toxin immunoassay is widely used for rapid diagnosis of Clostridium difficile-associated diarrhea. The aim of this study was to evaluate the performance of Tox A/B Quik Chek test (TECHLAB,...
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Evaluation of the VIDAS CDAB Kits for the Detection of the Clostridium difficile Toxins A and B

Kang JO, Shin BM, Han D, Choi TY

BACKGROUND: Since the emergence of variant Clostridium difficile strains that fail to produce detectable toxin A, diagnostic kits targeted to detect toxin A only showed a considerable rate of false...
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Validation of enzyme immunoassay for the quantitative measurement of human IgG antibodies specific for Haemophilus influenzae Type b capsular polysaccharide

Kim KH, Lim SY

PURPOSE: This study was conducted to validate enzyme immunoassay (EIA) for the quantitative measurement of human IgG antibodies specific for Haemophilus influenzae type b (Hib) capsular polysaccharide. METHOD: We evaluated specificity,...
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Comparisons of Latex Agglutination, Immunochromatography and Enzyme Immunoassay Methods for the Detection of Rotavirus Antigen

Lee SY, Hong JH, Lee SW, Lee M

BACKGROUND: Rotavirus is the most common cause of childhood gastroenteritis during winter season. Rapid, accurate diagnosis is essential for preventing severe complications of rotaviral gastroenteritis. The sensitivity and specificity...
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Characterization of a Toxin A-Negative, Toxin B-Positive Variant Strain of Clostridium difficile

Shin BM, Kuak EY

BACKGROUND: Clostridium difficile is one of the most important pathogens responsible for nosocomial diarrhea. Recently, we have frequently experienced culture positive, toxin A enzyme immunoassay negative strains. Therefore, we evaluated...
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Comparison of Two Enzyme Immunoassays for Clostridium difficile Toxin A

Yoo SJ, Kang JO, Oh HJ, Shin BM

BACKGROUND: Clostridium difficile is one of the most important pathogens responsible for nosocomial diarrhea. The disease is mediated by two toxins, designated as A and B; therefore, identification of the...
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