- Detection of hepatitis B virus DNA in serum by digoxigenin labeled DNA probe
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Kim SH, Baek WK, Suh MH, Kim JR, Shin DH
- KMID: 1688149
- J Korean Soc Microbiol.
- 1993 Aug;28(4):303-311.
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- Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) in paraffin-embedded sections
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Kim O
- KMID: 1094690
- J Vet Sci.
- 2004 Mar;5(1):71-73.
Two non-radioactive probes using digoxigenin or biotin were developed for detecting canine herpesvirus (CHV) and compared for their sensitivities by in situ hybridization (ISH) in formalin fixed, paraffin embedded sections,... -
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- A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid Leprosy without the Bacilli under the Fite Stain
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Choi HY
- KMID: 1964697
- Ewha Med J.
- 1996 Jun;19(2):193-197.
- doi: 10.12771/emj.1996.19.2.193
In situ PCR was applied to the paraffin embedded tissue section of two borderline tuberculoid patients which did not show acid fast bacilli on the Fite stain. In situ PCR... -
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- Improved Technique of Digoxigenin Labeled RNA in situ Hybridization
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Lee SK, Kim YS, Song IS, Lee SS, Lee YJ, Kim WH, Chi JG
- KMID: 2275639
- Korean J Pathol.
- 2001 Apr;35(2):98-110.
BACKGROUND: A practical RNA in situ hybridization method using digoxigenin labeled RNA probes is described in order to evaluate the technical difficulties and problems in RNA in situ hybridization. METHODS:... -
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- Study on the Expression of Vasoactive intestinal polypeptide, Vasopressin and Oxytocin mRNAs in the Rat Brain using Double in situ Hybridization Technique
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Park KH, Park HS, Yoo YB, Lee WJ, Hwang DH, Lee BL, Cha CI, Cho SS, Baik SH
- KMID: 1530286
- Korean J Anat.
- 1998 Dec;31(6):807-815.
The technique of in situ hybridization using synthetic oligonucleotides labelled by non-radioactive method was developed to localize vasoactive intestinal polypeptide, arginine-vasopressin and oxytocin mRNAs in the rat brain. Also double... -
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- Comparison Between HLA-DR Serological Typing and O1igotyping
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Wee JH, Jeong KC, Kim TG, Han JY, Kim JM
- KMID: 2146210
- Korean J Clin Pathol.
- 1997 Dec;17(6):1089-1099.
BACKGROUND: In renal transplantation, a good HLA-DR match Is associated with successive graft outcome. But due to a number of technical problems, reliable serological DR typing cannot always be obtained.... -
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- A Study on Prevalence of Premutation Sized FMR1 Gene Using Polymerase Chain Reaction
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Park W, Lee KJ, Choi EY
- KMID: 2007245
- J Korean Child Neurol Soc.
- 1999 Oct;7(1):42-47.
BACKGROUND: Fragile X syndrome is one of the most common causes of mental retardation. For its prevention, detection of premutation range CGG repeat in FMR1 gene is necessary. The aim... -
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- Expression of MUC5AC and MUC8 mRNA in human nasal mucosa
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Kim HU, Lee YH, Lee JG, Yoon JH
- KMID: 1923259
- Korean J Otolaryngol-Head Neck Surg.
- 2001 May;44(5):490-494.
BACKGROUND AND OBJECTIVES: MUC5AC is known to be a major secretory mucin in goblet cells of the mucosa of human lower respiratory tract. But in our preliminary study, we found... -
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- Sensitive and Specific Detection of Mycoplasma species by Consensus Polymerase Chain Reaction and Dot Blot Hybridization
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Hong S, Lee HA, Park SH, Kim O
- KMID: 2053663
- Lab Anim Res.
- 2011 Jun;27(2):141-145.
- doi: 10.5625/lar.2011.27.2.141
Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Many species of mycoplasmas are important pathogens that cause respiratory infection in laboratory animals and that are known to... -
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- A Sensitive and Specific PCR/dot Blot Hybridization assay for the Detection of Ovine Herpesvirus 2, a Gamma Herpesvirus
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Kim O, Yi SJ
- KMID: 1615428
- J Bacteriol Virol.
- 2003 Mar;33(1):87-92.
Polymerase chain reaction (PCR) provides a powerful technique for identifying viruses and studying the homology between viral nucleic acids. However, PCR assay has limitations in its susceptibility to contamination or... -
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- The Expression of Cytokines in the Airways from Patients with Bronchial Asthma
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Uh ST, Jeong SW, Lee SM, Kim HT, Kim YH, Park CS
- KMID: 2045046
- Tuberc Respir Dis.
- 1995 Feb;42(1):1-10.
- doi: 10.4046/trd.1995.42.1.1
BACKGROUND: It has been well known that bronchial asthma is a chronic inflammatory disorder. The "activation" of lymphocytes has a significant role in the pathogenesis of bronchial asthma. Among these... -
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- Restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolated from Taegu
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Jeon CH, Lee SC, Sohn JH, Ahn WS
- KMID: 2031024
- Korean J Clin Pathol.
- 1999 Oct;19(5):581-586.
BACKGROUND: To investigate epidemiology of a specific strain, and evaluate correlation between Mycobacterium tuberculosis restriction fragment length polymorphism (RFLP) and antimicrobial susceptibility, we studied about Mycobacterium tuberculosis RFLP isolated from... -
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- Expression of alpha 2A -adrenergic receptor mRNA in the dorsal root ganglia in a rat neuropathic pain model
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Lee YH, Kim WS, Chung K, Chung JM
- KMID: 2283033
- Korean J Phys Anthropol.
- 1997 Dec;10(2):181-189.
To investigate whether the change of alpha 2A -AR mRNA expression in the DRG is an underlying mechanism of sympathetically maintained neuropathic pain (SMP), in situ hybridization for alpha 2A... -
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- A Study of Standardization of the In situ PCR ( Polymerase Chain Reaction ) on the Tissues of Borderline Leprosy Patients
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Choi HY
- KMID: 2303555
- Korean J Dermatol.
- 1996 Jun;34(3):415-421.
BACKGROUND: PCR from paraffin-embedded tissues has been recently applied on the diagnosis of leprosy, PCR is a highly sensitive technique and the amplified product is detected by gel electrophoresis and... -
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- TNFalpha Expression in the Paraffin-embedded Tissues of Leprosy
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Lee JE, Choi YW, Choi HY, Myung KB
- KMID: 1863723
- Korean J Dermatol.
- 2000 Mar;38(3):363-371.
BACKGROUND: Tumor necrosis factor alpha (TNFalpha ) plays various roles in the pathogenesis of leprosy. Expression of TNFalpha according to the clinical type of leprosy has been studied in the patient... -
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- Early Detection of Human Cytomegalovirus DNA by PCR-ELISA
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Kim M, Yoon M, Lim WH, Shin JH, Suh SP, Ryang DW
- KMID: 2089540
- Korean J Clin Pathol.
- 1998 Sep;18(3):407-413.
BACKGROUND: Human cytomegalovirus (CMV) infections are common and occasionally severe in newborns, immunocompromised hosts, cancer patients, and recipients of organ transplant. Consequently, sensitive and rapid methods for CMV detection are... -
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- In situ Hybridization for the Detection and Localization of the Bitter Taste Receptor Tas2r108 in the Murine Submandibular Gland
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Ki SY, Cho YK, Chung KM, Kim KN
- KMID: 2328537
- Int J Oral Biol.
- 2016 Jun;41(2):97-103.
- doi: 10.11620/IJOB.2016.41.2.097
Mammals have 3 pairs of major salivary glands i.e., the parotid, submandibular, and sublingual glands. Saliva secretion of these glands is modulated by taste perception. Salivary glands are composed mainly... -
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- Expression of c-erbB2 and HLA-A2 in Breast Cancer Patients
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Lee HD, Koo JY, Kim DY, Jung WH, Jang YS, Park JH
- KMID: 2173609
- J Korean Breast Cancer Soc.
- 1999 Dec;2(2):152-158.
- doi: 10.4048/jkbcs.1999.2.2.152
PURPOSE: Oncogene c-erbB2 produces a transmembrane protein similar in structure to the tyrosine kinase family. Overexpression of c-erbB2 is known to lower the survival rate of breast cancer patients. c-erbB2... -
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- Detection and Quantification of Residual Cellular DNA in the Production of Recombinant HPV-16 L1 Virus-Like Particles
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Son HK, Jeong HS, Kim YL, Kim SN, Shin JH, Min HK, Seong BL, Park SN
- KMID: 2055036
- J Bacteriol Virol.
- 2006 Dec;36(4):271-278.
- doi: 10.4167/jbv.2006.36.4.271
A number of recombinant proteins isolated from cell sources are being produced for biopharmaceuticals. Although most biopharmaceuticals are highly purified, there is a safety concern that such recombinant products could... -
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- Renal Adaptive Responses of HK alpha2 Gene(HK alpha 2a, HK alpha 2b) to the Changes of Potassium(K) Diet
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Ahn KY, Kim BY, Lee SW, Kim KK, Park SS
- KMID: 2307300
- Korean J Nephrol.
- 1999 Sep;18(5):672-682.
Recent molecular and physiological studies suggested that at least two H/K-ATPase isozymes are expressed in the rat kidney, and two distinct isoforms(HK alpha 2a, 2b) are resulted from alternative splicing... -
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