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Clinical and Radiological Features of Korean Patients With Anti-HMGCR Myopathy

Oh EK, Lee SA, Lee HJ, Cha YJ, Kim S, Lee HS, Suh BC, Shin HY, Kim SW, Yoon BA, Oh Si, Kim YH, Cho JY, Cho JH, Kwon KH, Choi YC, Park HJ

Background and Purpose To understand the characteristics of Korean patients with anti-3-hydroxy-3-methylglutaryl-coenxyme A reductase (HMGCR) myopathy, we measured antiHMGCR antibodies and analyzed the clinical, radiological, and pathological features of patients...
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Facile Titrimetric Assay of Lysophosphatidic Acid in Human Serum and Plasma for Ovarian Cancer Detection

Tarannum N, Kumar D, Agrawal R

Herein, an instrument free facile acid-base titrimetric methodology is reported for lysophosphatidic acid (LPA) measurement in serum and plasma samples for ovarian cancer detection. The concept is based on the...
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Prevalence of bovine viral diarrhea virus from Korean native cattle farms in Jeju

Cho SC, Yang HS, Park C, Kim ST, Ko EJ, Son WG

Bovine viral diarrhea virus (BVDV) is an RNA virus belonging to Pestivirus in the family Flaviviridae. BVDV has economic significance for the livestock industry because of its association with acute...
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Measurement of serum anti-Müllerian hormone by revised Gen II or automated assay: Reproducibility under various blood/serum storage conditions

Lee JY, Kim CH, Choe SA, Seo S, Kim SH

Objective: We investigated the agreement between anti-Müllerian hormone (AMH) levels measured with revised Gen II (rev-Gen II) and automated AMH (Access) assays and evaluated the reproducibility of each method under...
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Therapeutic effects of paeoniflorin on irritable bowel syndrome in rats

Wang L, Lei J, Zhao Z, Jia J, Wang L

Background: Irritable bowel syndrome (IBS) is a functional bowel disorder (FBD). Objectives: To assess the therapeutic effects of paeoniflorin (PF) on IBS in rats. Method: Sixty male Sprague–Dawley rats were randomly divided...
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A Neuroprotective Action of Quercetin and Apigenin through Inhibiting Aggregation of Aβ and Activation of TRKB Signaling in a Cellular Experiment

Chiu YJ, Teng YS, Chen CM, Sun YC, Hsieh-Li HM, Chang KH, Lee-Chen GJ

Alzheimer’s disease (AD) is a neurodegenerative disease with progressive memory loss and the cognitive decline. AD is mainly caused by abnormal accumulation of misfolded amyloid β (Aβ), which leads to...
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A Survey on Antinuclear Antibodies Testing in Korea

Kim KH

Background: Antinuclear antibodies (ANA) detected by indirect immuno fluorescence assay (IFA) on HEp-2 cells are important for the diagnosis of systemic rheumatic diseases. ANA is an entry criterion in the...
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Development and Application of a Cell-Based Assay for LRP4 Antibody Associated With Myasthenia Gravis

Chung HY, Kim MJ, Kim SW, Oh J, Shin HY

Background and Purpose Among patients with double-seronegative myasthenia gravis (dSN-MG) who do not have detectable antibodies against acetylcholine receptor or musclespecific tyrosine kinase, autoantibodies against low-density lipoprotein receptor-related protein 4...
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A standardized method to study immune responses using porcine whole blood

Mattoo SuS, Aganja RP, Kim SC, Jeong CG, Nazki S, Khatun A, Kim WI, Lee SM

Background: Peripheral blood mononuclear cells (PBMCs) are commonly used to assess in vitro immune responses. However, PBMC isolation is a time-consuming procedure, introduces technical variability, and requires a relatively large...
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Virtual Screening and Testing of GSK-3 Inhibitors Using Human SH-SY5Y Cells Expressing Tau Folding Reporter and Mouse Hippocampal Primary Culture under Tau Cytotoxicity

Lin CH, Hsieh YS, Sun YC, Huang WH, Chen SL, Weng ZK, Lin TH, Wu YR, Chang KH, Huang HJ, Lee GC, Hsieh-Li HM, Lee-Chen GJ

Glycogen synthase kinase-3β (GSK-3β) is an important serine/threonine kinase that implicates in multiple cellular processes and links with the neurodegenerative diseases including Alzheimer’s disease (AD). In this study, structure-based virtual...
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Interlaboratory Comparison Study (Ring Test) of Next-Generation Sequencing–Based NTRK Fusion Detection in South Korea

Lee SE, Lee MS, Jeon YK, Shim HS, Kang J, Kim J, Choi YL

Purpose Tropomyosin receptor kinase (TRK) inhibitors are approved for the treatment of neurotrophic receptor tyrosine kinase (NTRK) fusion-positive tumors. The detection of NTRK fusion using a validated method is required...
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Allergic Sensitization Pattern in the Korean Dermatologic Patients

Han Y, Woo YR, Kim HS, Lee JD, Choi S, Yu J, Cho SH

Background: Avoiding causative allergens is important for controlling the clinical course of allergic diseases. Allergen sensitization is influenced by many factors including the environment and lifestyle. The socioeconomic development, climate,...
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Genotoxicity study of 2-methoxyethanol and benzalkonium chloride through Comet assay using 3D cultured HepG2 cells

Lim C, Shin K, Seo D

Though the key data in identifying carcinogenicity is experience in human, long-term carcinogenicity tests using experimental animals are more realistic. Because carcinogenicity tests require much time and cost, performing the...
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Re-Cellularised Kidney Scaffold for Chikungunya Virus Propagation: A Novel Approach

Walawalkar S, Almelkar S

BACKGROUND: Re-emerging viral attacks are catastrophic for health and economy. It is crucial to grasp the viral life cycle, replication and mutation policies and attack strategies. It is also absolute...
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Evaluation of the Xpert Carba-R Assay for Detection of Rectal Carbapenemase-producing Enterobacterales in a Korean Tertiary Hospital

Han J, Ha SI, Shin DP, Cha YJ, Yoo IY, Park YJ

Background: Carbapenemase-producing Enterobacterales (CPE) have been highlighted as an urgent threat by international health authorities. Rapid detection of carbapenemase will allow implementation of infection control measures. Methods: We assessed the performance...
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Applying Functional Assay Evidence to Interpret Sequence Variants Identified in Hereditary Cancer Genes

Choi YJ, Kim B, Kim Y, Shin S, Lee KA

The demand for the interpretation of sequence variants identified by next-generation sequencing is gradually increasing in clinical laboratories. The American College of Medical Genetics and the Association for Molecular Pathology...
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Commutability Assessment of Frozen Human Serum Pools for External Quality Assessment of Tumor Markers

Cho Ej, Kim SH, Hong J, Lee H, Hyun J, Cho SE, Lee W, Kim HS, Oh EJ, Chun S, Min WK

Background: Commutability is essential to harmonize different measuring systems and to evaluate the performance of participating laboratories in external quality assessment (EQA). This study aims to assess the com mutability...
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Chemical Profile and Cytotoxicity Activity of Stem-bark of Anacardium occidentale

Shehu A, Ponnapalli MG, Mahboob M, Prabhakar PV, Olatunji GA

Column chromatographic fractionation of the methanol and ethyl acetate extracts of the stem-bark of Anacardium occidentale led to the isolation of five compounds (1-5). Their structures were determined by spectroscopic...
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The relationship between reactive oxygen species, DNA fragmentation, and sperm parameters in human sperm using simplified sucrose vitrification with or without triple antioxidant supplementation

Juanpanich T, Suttirojpattana T, Parnpai R, Vutyavanich T

Objective: This study examined whether the addition of triple antioxidants (3A)—10 μM acetyl-L-carnitine, 10 μM N-acetyl-L-cysteine, and 5 μM α-lipoic acid—in freezing-thawing medium during human sperm cryopreservation using the sucrose...
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A combined application of molecular docking technology and indirect ELISA for the serodiagnosis of bovine tuberculosis

Song S, Zhang Q, Yang H, Guo J, Xu M, Yang N, Yi J, Wang Z, Chen C

Background: There is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components...
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