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Application of 16S rRNA Gene-Targeted Next-Generation Sequencing for Bacterial Pathogen Detection in Continuous Ambulatory Peritoneal Dialysis Peritonitis

Kim YA, Kang EW, Moon HS, Kim D, Yong D

BACKGROUND: 16S rRNA gene-targeted next-generation sequencing (NGS) can detect microorganisms in a comprehensive reference database. To date, NGS has been successfully applied to samples such as urine, blood, and synovial...
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A Case of Catabacter hongkongensis and Alistipes indistinctus Isolated from Blood Cultures of a Patient with Acute Appendicitis

Ryu J, Kim Y, Lee J, Cho SY, Park TS, Lee HJ

Catabacter hongkongensis is an anaerobic gram-positive coccobacillus that was first isolated in Hong Kong. It is infectious and causes high mortality in patients with rare but underlying diseases. Alistipes indistinctus...
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Facklamia hominis Isolated from a Wound: A Case Report and Review of the Literature

Kim TY, Jo J, Kim N, Park H, Roh EY, Yoon JH, Shin S

Facklamia hominis is a facultative anaerobic Gram-positive coccus generally displaying weak alpha-hemolysis and negativity for catalase and oxidase. Facklamia species are part of the normal flora of the female genitourinary...
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Molecular identification of coagulase-negative staphylococci by rpoB sequence typing

Seong WJ, Kim D, Kim EK, Ko DS, Ro Y, Kim JH, Kwon HJ

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has...
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Bacterial diversity and its relationship to growth performance of broilers

Bae Y, Koo B, Lee S, Mo J, Oh K, Mo IP

The microbial community is known to have a key role during the rearing period of broilers. In this study, gut microbial composition and diversity were examined to evaluate the relationships...
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Utility of a Direct 16S rDNA PCR and Sequencing for Etiological Diagnosis of Infective Endocarditis

Kim MS, Chang J, Kim MN, Choi SH, Jung SH, Lee JW, Sung H

BACKGROUND: Cases of infective endocarditis (IE) require prompt etiological diagnosis for effective treatment. Molecular methods can aid in rapid and reliable diagnosis of culture-negative IE cases. We evaluated the utility...
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Identification of streptococcus dysgalactiae subsp. equisimilis from septic knee by 16S rRNA gene sequencing

Park JS, Kim HW, Chae JD, Hur JW, Park YS, Yoo MS, Ryu HY

Septic arthritis is the infection of a joint by an infectious agent, leading to arthritis. It is therefore important to identify and treat the correct bacteria in septic arthritis. However,...
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Two Cases of Medical Device-Related Corynebacterium striatum Infection: A Meningitis and A Sepsis

Park S, Chung HS, Seo EK, Mun YC, Lee M

Corynebacterium striatum is a commonly isolated contaminant in the clinical microbiology. However, it can be an opportunistic pathogen in immunocompromised and even immunocompetent hosts. The increasing prevalence of C. striatum...
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Comparison of the Vitek 2, API 20A, and 16s rRNA Gene Sequencing for the Identification of Anaerobic Bacteria

Park GC, Jang SJ, Lee MJ, Kook JK, Kim MJ, Kim YS, Yang NW, Lee HS, Kang SH, Park G, Moon DS

BACKGROUND: Recently, genotypic identification of anaerobes is emerging as an alternative to the phenotypic method. In this study, we evaluated the performance of Vitek 2, API 20A and 16s rRNA...
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Detection of Anaplasma sp. in Korean Native Goats (Capra aegagrus hircus) on Jeju Island, Korea

Seong G, Han YJ, Chae JB, Chae JS, Yu DH, Lee YS, Park J, Park BK, Yoo JG, Choi KS

Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have...
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Sternal Osteomyelitis Caused by Gordonia bronchialis after Open-Heart Surgery

Chang JH, Ji M, Hong HL, Choi SH, Kim YS, Chung CH, Sung H, Kim MN

We report the case of a deep sternal wound infection with sternal osteomyelitis caused by Gordonia bronchialis after open-heart surgery. The isolate was identified as a G. bronchialis by 16S...
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A Case of Brain Abscess Caused by Propionibacterium acnes 13 Months after Neurosurgery and Confirmed by 16S rRNA Gene Sequencing

Chung S, Kim JS, Seo SW, Ra EK, Joo SI, Kim SY, Park SS, Kim EC

Propionibacterium acnes is a gram-positive anaerobic bacillus and a normal inhabitant of the skin. Although it is often considered a contaminant of blood cultures, it can occasionally cause serious infections,...
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First Isolation of Streptococcus gallolyticus subsp. pasteurianus from a Korean Patient with Severe Septic Shock

Jeong S, Park JY, Han SH, Lee Y, Yong D, Lee K, Chong Y

A 60-year-old man presented with a 1-day history of fever, vomiting, and diarrhea. He was diagnosed with severe septic shock on the basis of a body temperature of 38.9degrees C,...
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Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing

Lim SM, Lee TK, Kim EJ, Park JH, Lee Y, Bae KS, Kum KY

OBJECTIVES: The purpose of this in vivo study was to investigate the microbial diversity in symptomatic and asymptomatic canals with primary endodontic infections by using GS FLX Titanium pyrosequencing. MATERIALS AND...
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Culture and Polymerase Chain Reaction of Helicobacter pylori from Rectal and Terminal Ileal Fluid after Polyethylene Glycol (Colyte(R)) Ingestion in Healthy Adults with Positive Urea Breath Test

Kim DH, Jung HM, Hwang YJ, Ahn YS, Mun JS, Myoung BH, Park H, Jeong EJ, Im YM, Oh HM, Jeong HY, Park C, Kim HR, Cho EH, Kim HD, Jung YD

BACKGROUND/AIMS: Helicobacter pylori (H. pylori) transmission route is not yet clearly understood. Isolating H. pylori from stool, saliva, and vomitus is very difficult. However, H. pylori could be cultured from...
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Molecular Identification of Clinical Rothia Isolates from Human Patients: Proposal of a Novel Rothia Species, Rothia arfidiae sp. nov.

Ko KS, Lee MY, Park YK, Peck KR, Song JH

Four Gram-positive cocci were isolated from the cerebrospinal fluid or blood of four different patients, but they could not be identified by an automated conventional identification system, so they were...
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Occurrence of Internal Stipe Necrosis of Cultivated Mushrooms (Agaricus bisporus) Caused by Ewingella americana in Korea

Lee CJ, Jhune CS, Cheong JC, Yun HS, Cho WD

The internal stipe necrosis of cultivated mushrooms (Agaricus bisporus) is caused by the bacterium Ewingella americana, a species of the Enterobacteriaceae. Recently, Ewingella americana was isolated from cultivated white button...
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Pseudooutbreak of Acinetobacter spp. Bacteriuria Confirmed by 16S rRNA Gene Sequence Analysis

Kim SY, Kim JY, Kang JH, Park SY, Lee HS, Park YS, Seo YH, Cho YK

  • KMID: 1528885
  • Infect Chemother.
  • 2007 Aug;39(4):208-212.
BACKGROUND: Acinetobacter spp. is increasingly implicated in hospital-acquired infections. We experienced a pseudooutbreak of Bordetella bronchiseptica bacteriuria identified with biochemical tests, that was later identified as Acinetobacter spp. by using...
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Isolation of Mycobacterium lentiflavum from a Patient with a Lung Destroyed by Tuberculosis

Shin S, Yoon JH, Song SH, Kim EC

Mycobacterium lentiflavum has recently been described as an emerging human pathogen without regard to the immune status of the host. We herein report on M. lentiflavum isolated from a respiratory...
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Antibiotic susceptibility of bacteria isolated from maxillary sinusitis lesion

Choi YO, Kim SG, Kim HK, Kim YJ, Choi DK, Kim MK, Park SN, Kim MJ, Kook JK

  • KMID: 1961036
  • J Korean Assoc Oral Maxillofac Surg.
  • 2006 Oct;32(5):436-446.
The purpose of this study was to isolate and identify the bacteria in chronic maxillary sinusitis (CMS) lesions from 3 patients and to determine the antimicrobial susceptibility of them against...
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