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The Efficient Transformation of Pleurotus ostreatus using REMI Method

Joh JH, Kim BG, Chu KS, Kong WS, Yoo YB, Lee CS

Restriction enzyme-mediated integration (REMI) was used to transform uracil auxotrophs of Pleurotus ostreatus to prototrophy. When protoplasts of Pleurotus ostreatus were treated by the reaction mixture containing 10 units of...
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Isolation and Characterization of Dikaryotic Mutants from Pleurotus ostreatus by UV Irradiation

Joh JH, Kim BG, Kong WS, Yoo YB, Chu KS, Kim NK, Park HR, Cho BG, Lee CS

Protoplasts of the wild type strain of Pleurotus osteatus were mutagenized with UV light, and 3,000 colonies were examined for abnormal mycelial and fruiting phenotypes. Forty one strains displayed variant...
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