Korean J Infect Dis.
1997 Mar;29(2):93-103.
Characterization of Extended-spectrum Beta-lactamases in Klebsiella pneumoniae Isolated in Korea
- Affiliations
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- 1Department of Internal Medicine, Dankook University College of Medicine.
- 2Department of Microbiology, Dankook University College of Medicine.
- 3Department of Clinical Pathology, Yonsei University college of Medicine.
- 4Department of Clinical Pathology, Seoul National University College of Medicine.
- 5Department of Microbiology, Kyungpook National University School of Medicine.
Abstract
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BACKGROUND: Extended-spectrum beta-lactamases (ESBLs) are plasmid-mediated enzymes that confer resistance to oxyimino-beta-lactams such as cefotaxime, ceftazidime, and aztreonam, antibiotics that were designed to be effective against strains producing known plasmid-determined beta-lactamases.
METHODS
Fifty-seven isolates of ESBL producing K. pneumoniae which were collected from 3 hospitals in Korea were characterized. They either transferred ceftazidime resistance or showed clavulanic acid enhancement of oxyimino-beta-lactam susceptibility. Beta-lactamase production by clinical isolates and transconjugants were characterized by isoelectric focusing and MIC values of oxyimino-beta-lactam were determined by agar dilution method. We performed PCR and RFLP for further characterization of TEM-type ESBLs.
RESULTS
The results showed that SHV-type ESBLs, especially SHV-5 and SHV-2 predominated, but TEM-4 and plasmid- mediated AmpC beta-lactamases (pI 8.0 and pI 8.4) were present as well: SHV-5, 26; SHV-2, 12; TEM-4, 9; plasmid-mediated AmpC type beta-lactamase (pI 8.0), 4; plasmid-mediated AmpC type beta-lactamase (pI 8.4), 1. Two isolates produced both TEM-4 and SHV-5, which showed higher MIC50 of cefotaxime, ceftazidime and aztreonam than those in the isolates producing TEM-4 or SHV-5. Three isolates produced both plasmid-mediated AmpC type beta-lactamases and other ESBLs, each one of TEM-4, SHV-2 and SHV-5. All of the isolates showed high resistance to cefoxitin, cefotaxime, ceftazidime and aztreonam.
CONCLUSION
With these results we conclude that SHV-type ESBLs outnumber and plasmid-mediated AmpC type beta-lactamases are present in high frequency in Korea.
MeSH Terms
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Agar
Anti-Bacterial Agents
Aztreonam
beta-Lactamases*
Cefotaxime
Cefoxitin
Ceftazidime
Clavulanic Acid
Isoelectric Focusing
Klebsiella pneumoniae*
Klebsiella*
Korea*
Pneumonia
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Agar
Anti-Bacterial Agents
Aztreonam
Cefotaxime
Cefoxitin
Ceftazidime
Clavulanic Acid
beta-Lactamases
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