In the present study, 5% Nanoxidil(R) was applied to C57BL/6 mice, and the expressions of IGF-1 and TGF-beta1 were quantitatively measured through PCR analysis. There were three groups; normal saline (negative control group), 50% EtOH (vehicle control group) and 5% Nanoxidil(R) (experimental group). The topical treatment of 5% Nanoxidil(R) for 2 weeks to dorsal skin accelerated hair regrowth more than other groups: In 5% Nanoxidil(R) group, the expression of IGF-1 was significantly increased compared with normal saline and 50% EtOH groups. Compared with normal saline group, the expression in 50% EtOH group was increased by about 1.6 times and by about 2.5 times in 5% Nanoxidil(R) group compared with the normal saline group. Conversely, the expression of TGF-beta1 was the lowest in 5% Nanoxidil(R) group and the highest in normal saline group. The expression of IGF-1, which stimulates hair growth, was found to be more in experimental group than in negative control group, whereas the expression of TGF-beta1, which inhibits hair growth, was found to be less in experimental group. Thus, the results showed that stimulation of hair growth with 5% Nanoxidil(R) produced an increased IGF-1 expression and decreased TGF-beta1 expression.