Korean J Lab Med.  2008 Jun;28(3):214-220. 10.3343/kjlm.2008.28.3.214.

Evaluation of Two Automated Instruments for Pre-transfusion Testing: AutoVue Innova and Techno TwinStation

• Shin SY
• Kwon KC
• Koo SH
• Park JW
• Ko CS
• Song JH
• Sung JY

• KMID: 854870
• DOI: http://doi.org/10.3343/kjlm.2008.28.3.214

Abstract

BACKGROUND
Despite the advances in total laboratory automation, a considerable amount of work in blood banks is still done using outdated manual methods. Some automated pre-transfusion testing instruments have recently been developed. Of these, we evaluated and compared the AutoVue Innova (Ortho, USA) and the Techno TwinStation (DiaMed AG, Switzerland).
METHODS
Forward and reverse ABO/Rh typing and unexpected antibody screening and identification tests were performed on 4,628 samples using the manual method and the two automated instruments. Two different anticoagulants (EDTA and citrate) were compared in ABO/Rh typing and unexpected antibody screening tests. Titrating studies were conducted on the following 7 dilutions using 5 samples of irregular antibodies with anti-E, anti-E & -c, anti-D, and anti-Le(a) with anti-Fy(a): 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, and 1:128. The test throughput per hour, the time required to perform 1 and 100 tests, and a simulation test for total events occurring in 1 day were also measured.
RESULTS
No erroneous results were reported between the two instruments and the manual method. Discrepancies observed in 10 cases (0.4%) of ABO/Rh typing were of higher intensity with AutoVue Innova than with the manual method. AutoVue Innova exhibited the highest sensitivity in the titrating study and throughput performance compared with the manual method and the Techno TwinStation. Especially in the throughput and time required to complete 100 antibody screening tests, AutoVue Innova had a 3.3- and 3.5-fold higher performance, respectively, than Techno TwinStation.
CONCLUSIONS
Because both of the two fully automated instruments (AutoVue Innova and Techno TwinStation) had high levels of accuracy and performance, it is expected that use of fully automated instruments will reduce human labor, turnaround time, and operator error in the blood bank.

Keyword

AutoVue Innova; Techno TwinStation; Pre-transfusion test

MeSH Terms

ABO Blood-Group System/blood
Antibodies/blood
Automation
Blood Grouping and Crossmatching/*instrumentation
Blood Transfusion
Cost-Benefit Analysis
False Positive Reactions
Humans
Rh-Hr Blood-Group System/blood

Reference

1.Mcpherson RA, Pincus MR, editors. Henry's clinical diagnosis and management by laboratory methods. 21th ed.Philadelphia: Saunders;2007. p. 58.

2.Recommendations for evaluation, validation and implementation of new techniques for blood grouping, antibody screening and cross-matching. British Committee for Standards in Haematology, Blood Transfusion Task Force. Transfus Med. 1995. 5:145–50.

3.Han KS, park MH, editors. Transfusion medicine. 3rd ed.Seoul: Korea Medical Book Pubisher Co;2006. p. 360. (한규섭, 박명희, 등. 수혈의학. 제3판. 서울: 고려의학, 2006;360.).

4.Burtis CA, Ashwood ER, editors. Tietz textbook of clinical chemistry and molecular diagnostics. 4th ed.Philadelphia: Saunders;2006. p. 492–3.

5.Morelati F., Revelli N., Maffei LM., Poretti M., Santoro C., Parravicini A, et al. Evaluation of a new automated instrument for pretransfusion testing. Transfusion. 1998. 38:959–65.

6.Han KS., Oh WI., Park MH., Kim EJ., Kim SI. Irregular blood group antibodies in Korean. Korean J Hematol. 1989. 24:145–53. (한규섭, 오원일, 박명희, 김의종, 김상인. 한국인의 혈액형 불규칙항체에 관한 연구. 대한혈액학회지 1989;24: 145-53.).