Yonsei Med J.  2007 Jun;48(3):495-501. 10.3349/ymj.2007.48.3.495.

Expression of Cyclooxygenase-2 and its Relationship to p53 Accumulation in Colorectal Cancers

Affiliations
  • 1Research Center for Resistant Cells, Chosun University College of Medicine, Gwangju, Korea.
  • 2Department of Preventive Medicine, Chosun University College of Medicine, Gwangju, Korea.
  • 3Department of Surgery, Chosun University College of Medicine, Gwangju, Korea. kjkim@chosun.ac.kr

Abstract

PURPOSE
Cyclooxygenase (COX)-2 is an inducible isoform responsive to cytokines, mitogens, and growth factors, and is believed to be an important enzyme related to colorectal cancer (CRC). Existing evidence suggests that COX-2 expression is normally suppressed by wild-type p53 but not mutant p53, suggesting that loss of p53 function may result in the induction of COX-2 expression. The aim of this study was to determine the relationship between COX-2 expression and p53 levels in CRC. MATERIALS AND METHODS: Patients with sporadic colorectal adenocarcinoma (n=161) who underwent curative surgery in Chosun University Hospital were enrolled in this study. Expression of COX-2 and p53 proteins was examined by immunohistochemistry in paraffin-embedded cancer tissue blocks, and the relationship between COX-2 and/or p53 expression with clinicopathologic parameters was analyzed. RESUTLS: Expression of COX- 2 was positive in 47.8% of colorectal cancers, and significantly associated with the depth of tumor invasion (p= 0.042). In contrast, p53 was positive in 50.3% of the cases, and was associated with both age (p=0.025) and the depth of tumor invasion (p=0.014). There was no correlation between COX-2 expression and p53 expression (p=0.118). CONCLUSION: These results suggest that COX-2 expression might play an important role in the progression of colorectal cancer. However, COX-2 expression was not associated with mutational p53. Further studies are needed to clarify the regulatory mechanisms governing COX-2 overexpression in colorectal cancers.

Keyword

Cyclooxygenase-2; p53; colorectal cancer; immunohistochemistry

MeSH Terms

Adenocarcinoma/*metabolism/pathology/surgery
Aged
Colorectal Neoplasms/*metabolism/pathology/surgery
Cyclooxygenase 2/*metabolism
Female
Humans
Immunohistochemistry
Male
Middle Aged
Mutation
Tumor Suppressor Protein p53/genetics/*metabolism

Figure

  • Fig. 1 Immunohistochemical staining for COX-2 shown in a representative section. (A) Negative COX-2 staining. (B) Positive staining for COX-2 in colorectal cancer cells.

  • Fig. 2 Immunohistochemical staining for p53 shown in a representative section. (A) Negative staining (less than 10%) for p53. (B) Positive staining for p53.


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