Yonsei Med J.  2000 Jun;41(3):381-386. 10.3349/ymj.2000.41.3.381.

Emerging antimicrobial resistance, plasmid profile and pulsed-field gel electrophoresis pattern of the endonuclease-digested genomic DNA of Neisseria gonorrhoeae

Affiliations
  • 1Department of Clinical Pathology, Yonsei University College of Medicine, Seoul, Korea. leekcp@yumc.yonsei.ac.kr
  • 2Department of Clinical Pathology, Soonchunhyang University, College of Medicine, Seoul, Korea.
  • 3Research Institute of Bacterial Resistance, Yonsei University, College of Medicine, Seoul, Korea.
  • 4National Institute of Health, Seoul, Korea.

Abstract

Resistant gonococci are very prevalent in many countries, particularly in Asia. This study was conducted to determine the trend of resistance, the effect of decreasing the ciprofloxacin susceptibilities of gonococci on the prevalence of penicillinase-producing N. gonorrhoeae (PPNG), and to compare the epidemiology of strains with the previous studies. A total of 602 strains of gonococci were isolated from prostitutes in 1997-1999. Antimicrobial susceptibility was tested by NCCLS disk diffusion and agar dilution methods. For epidemiologic analysis, vplasmid analysis and pulsed-field gel electrophoresis (PFGE) were performed. The proportion of PPNG remained high (79%), and the strains with decreased susceptibility to ciprofloxacin increased significantly from 67% in 1997 to 84% in 1999. Compared to our previous study, the PFGE patterns were similar, while the proportion of strain with the 3.2-MDa plasmid markedly decreased. In conclusion, a rapid increase in ciprofloxacin-nonsusceptible strains may suggest difficulties in the treatment of gonococcal infections in the near future with the drug. The recent decrease of PPNG with the 3.2-MDa plasmid may suggest that there is an epidemiological change in gonococcal infections, and the prevalence of related PFGE patterns suggests the dissemination of a few clones among the high risk populations.

Keyword

Neisseria gonorrhoeae; a-lactamase; ciprofloxacin resistance; plasmid; pulsed-field gel electrophoresis

MeSH Terms

DNA/genetics*
DNA/drug effects
Drug Resistance, Microbial/genetics*
Electrophoresis, Gel, Pulsed-Field
Endonucleases/pharmacology
Female
Human
Neisseria gonorrhoeae/genetics*
Plasmids/genetics*

Cited by  2 articles

Increasing Incidence of High-Level Tetracycline-Resistant Neisseria gonorrhoeae due to Clonal Spread and Foreign Import
Hyukmin Lee, Hyunsoo Kim, Hyo Jin Kim, Young Hee Suh, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee, Yunsop Chong
Yonsei Med J. 2016;57(2):350-357.    doi: 10.3349/ymj.2016.57.2.350.

Antimicrobial Resistance of Neisseria gonorrhoeae Isolated in Korea
Hyukmin Lee, Kyungwon Lee, Yunsop Chong
J Bacteriol Virol. 2012;42(1):9-16.    doi: 10.4167/jbv.2012.42.1.9.

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