Lab Med Online.  2022 Oct;12(4):262-268. 10.47429/lmo.2022.12.4.262.

Body Fluid Analysis for Cellular Composition Using Manual Methods: Current Status and Clinical Laboratory Guidelines in Korea (2021)

Affiliations
  • 1Department of Laboratory Medicine, Soonchunghyang University Seoul Hospital, Seoul, Korea
  • 2Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
  • 3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea
  • 4Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan, Korea
  • 5Department of Laboratory Medicine, Pusan National University Yangsan Hospital, Yangsan, Korea
  • 6Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea
  • 7Department of Laboratory Medicine, Chungnam National University College of Medicine, Daejeon, Korea
  • 8Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea
  • 9Department of Laboratory Medicine, Haeundae Paik Hospital, Inje University College of Medicine, Busan, Korea
  • 10Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Korea
  • 11Department of Laboratory Medicine, National Cancer Center Hospital, Goyang, Korea

Abstract

To investigate the current status of body fluid analysis for cellular composition in Korea, an online survey was performed. Responses were received from 76 laboratories of general hospitals in Korea. The questionnaire included questions on pre-analytical, analytical, and post-analytical processes of body fluid cellular analysis (cell count using hemocytometers and cytospin) performed in each laboratory. The survey showed that the body fluid cellular analysis in Korea is not harmonized. Based on this, the “Committee for Standardization in Korean Society for Laboratory Hematology” suggests clinical laboratory guidelines for the pre-analytical (sample collection and handling), analytical (cell count, slide preparation), post-analytical (reporting, turnaround time), and quality control steps. We hope that the results of this survey and guideline will serve as a basis for setting future standards of body fluid cellular analysis in Korea, and ultimately have a positive impact on patient care.

Keyword

Questionnaire survey; Guideline; Body fluid; Body fluid cellular analysis

Figure

  • Fig. 1 Number of body fluid cellular analysis tests per month; of various sample types from 76 clinical laboratories in Korea.

  • Fig. 2 Methods used for body fluid cellular analysis in 76 clinical laboratories in Korea.

  • Fig. 3 Schematic diagram of hemocytometer. The hemocytometer is composed of nine large squares, and four squares (labeled as 1, 3, 7, and 9) in the corners are divided into 16 smaller squares. The central square (labeled as 5) is divided into 25 smaller squares, and each is further divided into 16 smaller squares.


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