Nat Prod Sci.  2022 Dec;28(4):181-186. 10.20307/nps.2022.28.4.181.

Structure Determination of Heishuixiecaoline A from Valeriana fauriei and Its Content from Different Cultivated Regions by HPLC/PDA Analysis

Affiliations
  • 1Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea
  • 2School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea
  • 3Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 27709, Republic of Korea
  • 4Department of Clinical Pharmacology, College of Medicine, Soonchunhyang University, Cheonan 31151, Republic of Korea
  • 5Department of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
  • 6BET Research Institute, Chung-Ang University, Anseong 17546, Republic of Korea
  • 7Natural Product Institute of Science and Technology, Anseong 17546, Republic of Korea

Abstract

A germacrane-type sesquiterpenoid was isolated and purified from a methanol extract of the roots of Valeriana fauriei (RVF) through open column chromatography using silica gel. This compound was verified to be heishuixiecaoline A by spectroscopic analysis. This compound was isolated for the first time from RVF. Quantitative analysis of heishuixiecaoline A from RVF cultivated from three different regions (Eumseong, Jinbu, and Jinan regions) was performed by combining high-performance liquid chromatography with a photodiode array detector. The extract of RVF cultivated in the Jinbu region showed the highest content (9.23 mg/g). In addition, a significant amount of the compound was detected in all RVF samples, which could be expected since it is a characteristic compound of RVF. The sesquiterpenoid group heishuixiecaoline A was isolated from RVF, a resource for various pharmacological substances, and quantitative analysis of RVF cultivated from three different regions was performed. As a result of these experiments, basic data on RVF that can be used in the development and application of pharmaceuticals and health functional foods in the future were obtained.

Keyword

Valeriana fauriei; heishuixiecaoline A; cultivation; HPLC
Full Text Links
  • NPS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr